Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2853281..2853864 Replicon chromosome
Accession NZ_LM995446
Organism Escherichia coli strain K-12 substr. RV308

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag ECRV308_RS14190 Protein ID WP_000254738.1
Coordinates 2853281..2853616 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag ECRV308_RS14195 Protein ID WP_000581937.1
Coordinates 2853616..2853864 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRV308_RS14175 2849168..2850466 - 1299 WP_000036723.1 phosphopyruvate hydratase -
ECRV308_RS14180 2850554..2852191 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
ECRV308_RS14185 2852419..2853210 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
ECRV308_RS14190 2853281..2853616 - 336 WP_000254738.1 endoribonuclease MazF Toxin
ECRV308_RS14195 2853616..2853864 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
ECRV308_RS14200 2853942..2856176 - 2235 WP_000226815.1 GTP pyrophosphokinase -
ECRV308_RS14205 2856224..2857525 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T285444 WP_000254738.1 NZ_LM995446:c2853616-2853281 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT285444 WP_000581937.1 NZ_LM995446:c2853864-2853616 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References