285442

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2708446..2709883 Replicon chromosome
Accession NZ_LM995446
Organism Escherichia coli strain K-12 substr. RV308

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag ECRV308_RS13440 Protein ID WP_000155570.1
Coordinates 2708446..2709519 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag ECRV308_RS13445 Protein ID WP_000461704.1
Coordinates 2709512..2709883 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRV308_RS13425 2703879..2706068 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
ECRV308_RS13430 2706065..2707681 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
ECRV308_RS13435 2708041..2708304 - 264 WP_000502050.1 DUF1524 domain-containing protein -
ECRV308_RS13440 2708446..2709519 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
ECRV308_RS13445 2709512..2709883 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
ECRV308_RS13450 2710238..2711101 + 864 WP_000787597.1 GTPase family protein -
ECRV308_RS13455 2711193..2712014 + 822 WP_000197391.1 DUF945 domain-containing protein -
ECRV308_RS13460 2712231..2712932 + 702 WP_000189409.1 WYL domain-containing protein -
ECRV308_RS13465 2712973..2713209 + 237 WP_001144029.1 hypothetical protein -
ECRV308_RS13470 2713209..2713652 + 444 WP_000824222.1 hypothetical protein -
ECRV308_RS13475 2713676..2714143 + 468 WP_000702495.1 hypothetical protein -
ECRV308_RS13480 2714368..2714682 - 315 WP_000065803.1 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T285442 WP_000155570.1 NZ_LM995446:2708446-2709519 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT285442 WP_000461704.1 NZ_LM995446:2709512-2709883 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
AlphaFold DB A0A373F4G4

References