Detailed information of TA system TA028541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 5493031..5493604
• Replicon: chromosome
• Accession: NC_019738
• Organism: Microcoleus sp. PCC 7113

Toxin (Protein)

• Gene name: relE
• Uniprot ID: K9WIY1
• Locus tag: MIC7113_RS22580
• Protein ID: WP_015184505.1
• Coordinates: 5493302..5493604 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: K9WKQ9
• Locus tag: MIC7113_RS22575
• Protein ID: WP_015184504.1
• Coordinates: 5493031..5493318 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MIC7113_RS22550	5488597..5488887	+	291	WP_155898078.1	hypothetical protein	-
MIC7113_RS22555	5489339..5489905	+	567	WP_015184500.1	hypothetical protein	-
MIC7113_RS22560	5489941..5490231	+	291	WP_015184501.1	hypothetical protein	-
MIC7113_RS22565	5490386..5491666	-	1281	WP_015184502.1	hypothetical protein	-
MIC7113_RS22570	5491918..5492934	+	1017	WP_015184503.1	hypothetical protein	-
MIC7113_RS22575	5493031..5493318	+	288	WP_015184504.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
MIC7113_RS22580	5493302..5493604	+	303	WP_015184505.1	Txe/YoeB family addiction module toxin	Toxin
MIC7113_RS22585	5493669..5494448	-	780	WP_015184506.1	PRC-barrel domain-containing protein	-
MIC7113_RS22590	5494756..5495106	-	351	WP_015184507.1	hypothetical protein	-
MIC7113_RS22595	5495471..5496157	-	687	WP_015184508.1	diheme cytochrome C	-
MIC7113_RS22600	5496174..5497082	-	909	WP_015184509.1	MoxR family ATPase	-
MIC7113_RS22605	5497139..5498185	-	1047	WP_015184510.1	bifunctional riboflavin kinase/FAD synthetase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (21-100)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-73)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11996.73 Da
• Isoelectric Point: 8.4689

>T28541 WP_015184505.1 NC_019738:5493302-5493604 [Microcoleus sp. PCC 7113]
MAKKDKNKQQPEDTPVRDAVFHPEFREDLVFWIETDRKTALRAFKLIEAIMREPFTGIGKPELLKYLDSGAWSRRLTQEH
RIVYLVSDDRIDFLQARYHY

Nucleotide

• Length: 303 bp

>T28541 NC_019738:5493302-5493604 [Microcoleus sp. PCC 7113]
TTGGCGAAGAAGGATAAGAATAAACAACAACCTGAAGACACTCCAGTTCGAGATGCTGTCTTTCATCCAGAGTTTCGAGA
AGATCTGGTTTTCTGGATAGAGACAGACCGCAAGACTGCCCTGAGAGCCTTTAAACTAATTGAGGCGATCATGCGTGAGC
CTTTTACGGGAATTGGCAAGCCTGAGCTGCTGAAATACTTAGACTCAGGTGCATGGTCACGACGACTCACACAGGAACAC
CGCATTGTTTACTTGGTCAGTGATGACCGAATTGATTTCCTCCAAGCTCGATATCATTACTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10332.66 Da
• Isoelectric Point: 4.1683

>AT28541 WP_015184504.1 NC_019738:5493031-5493318 [Microcoleus sp. PCC 7113]
MPNQTSFTEAFANLAQLCDQVVADRDVVIITRQDGESVALIAADELDSLMETAYLLRSPTNAARLLTALQRAKTRTLEPQ
SISALRQEIGLGEEG

Nucleotide

• Length: 288 bp

>AT28541 NC_019738:5493031-5493318 [Microcoleus sp. PCC 7113]
ATGCCCAACCAAACATCCTTCACTGAGGCTTTCGCCAATCTTGCCCAACTCTGTGACCAAGTAGTTGCCGATCGGGATGT
GGTCATAATTACCCGACAGGACGGTGAAAGCGTGGCTCTCATTGCGGCTGATGAACTCGACAGCTTGATGGAGACAGCCT
ATTTACTGCGATCGCCCACAAATGCAGCTCGTCTATTAACTGCTCTGCAACGAGCGAAAACAAGAACACTAGAACCTCAA
AGCATCAGCGCCCTACGCCAGGAGATAGGACTTGGCGAAGAAGGATAA

Structures

Toxin

• AlphaFold DB: K9WIY1

Antitoxin

• AlphaFold DB: K9WKQ9