Detailed information of TA system TA028540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 233062..233773
• Replicon: chromosome
• Accession: NC_019738
• Organism: Microcoleus sp. PCC 7113

Toxin (Protein)

• Gene name: tad
• Uniprot ID: K9W8L2
• Locus tag: MIC7113_RS00995
• Protein ID: WP_015180305.1
• Coordinates: 233062..233460 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: MIC7113_RS01000
• Protein ID: WP_015180306.1
• Coordinates: 233444..233773 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MIC7113_RS00975	228468..229730	-	1263	WP_015180301.1	branched-chain amino acid ABC transporter substrate-binding protein	-
MIC7113_RS00985	230599..231867	+	1269	WP_041779844.1	glycosyltransferase family 1 protein	-
MIC7113_RS00990	231945..232793	-	849	WP_015180303.1	mechanosensitive ion channel family protein	-
MIC7113_RS35775	232833..233003	-	171	WP_155897892.1	hypothetical protein	-
MIC7113_RS00995	233062..233460	+	399	WP_015180305.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MIC7113_RS01000	233444..233773	+	330	WP_015180306.1	helix-turn-helix domain-containing protein	Antitoxin
MIC7113_RS35780	233776..233919	+	144	WP_155897893.1	hypothetical protein	-
MIC7113_RS32910	233928..234485	-	558	WP_015180307.1	NYN domain-containing protein	-
MIC7113_RS01010	234722..235249	-	528	WP_015180308.1	AbiV family abortive infection protein	-
MIC7113_RS01015	235556..236776	-	1221	WP_015180310.1	alanine racemase	-
MIC7113_RS34370	236921..237415	-	495	WP_071884083.1	DUF4114 domain-containing protein	-
MIC7113_RS01025	237330..238736	-	1407	WP_015180312.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-89)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14913.86 Da
• Isoelectric Point: 6.4872

>T28540 WP_015180305.1 NC_019738:233062-233460 [Microcoleus sp. PCC 7113]
MSSLLKPVEWVGSSLEDLKDFPEEVQQVVGYALYLAQCGEKHPSAKPLKGFKGARVLEVVEDFDGDTYRTVYTVKLAGVI
YVLHAFQKKSKQGIATPKQDIDLIETRLKRAQEHYAENYHKQQKGEENDSGN

Nucleotide

• Length: 399 bp

>T28540 NC_019738:233062-233460 [Microcoleus sp. PCC 7113]
ATGAGTTCATTGTTGAAACCAGTTGAATGGGTTGGAAGCTCCCTTGAGGATTTGAAAGATTTTCCAGAGGAGGTTCAGCA
AGTCGTTGGCTATGCCTTGTACTTGGCTCAATGCGGTGAGAAGCATCCTTCAGCTAAACCACTCAAGGGATTTAAGGGTG
CTAGGGTGCTTGAAGTTGTGGAAGACTTCGATGGAGATACTTACCGAACTGTTTACACGGTGAAACTTGCTGGCGTAATT
TATGTGTTGCACGCCTTCCAAAAGAAATCAAAGCAAGGAATCGCTACGCCAAAGCAGGATATTGATTTGATTGAAACAAG
ACTTAAACGAGCACAAGAGCATTACGCAGAAAACTATCACAAGCAGCAAAAGGGAGAGGAAAATGACTCAGGAAATTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11828.70 Da
• Isoelectric Point: 10.7684

>AT28540 WP_015180306.1 NC_019738:233444-233773 [Microcoleus sp. PCC 7113]
MTQEIRVQASSGNVFADLGLENSDELLVKAELARKISSIITKQGMTQAEAAELLGIDQPKVSALINGKLSGFSTVRLFRF
LNALGRDVEIVVKTKPKSRSQARTRVVAT

Nucleotide

• Length: 330 bp

>AT28540 NC_019738:233444-233773 [Microcoleus sp. PCC 7113]
ATGACTCAGGAAATTAGAGTGCAAGCAAGTAGCGGTAATGTGTTTGCCGATTTGGGTTTAGAAAATTCGGATGAATTGCT
TGTGAAAGCTGAACTCGCACGTAAGATCAGCAGCATTATTACTAAGCAAGGCATGACGCAGGCTGAAGCCGCAGAGCTTT
TAGGGATTGACCAGCCAAAAGTATCTGCTCTAATCAACGGCAAGTTATCAGGCTTTTCAACTGTACGGTTGTTTCGTTTC
TTGAATGCTTTGGGTCGAGATGTGGAAATTGTAGTGAAAACCAAACCTAAATCTCGCTCACAAGCTCGAACACGGGTTGT
TGCTACGTGA

Structures

Toxin

• AlphaFold DB: K9W8L2