Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 479313..479931 Replicon chromosome
Accession NZ_LM993812
Organism Escherichia coli strain K-12 substr. HMS174

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECHMS174_RS02365 Protein ID WP_001291435.1
Coordinates 479313..479531 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECHMS174_RS02370 Protein ID WP_000344800.1
Coordinates 479557..479931 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECHMS174_RS02335 474602..475174 + 573 WP_000779842.1 YbaY family lipoprotein -
ECHMS174_RS02340 475205..475516 - 312 WP_000409911.1 MGMT family protein -
ECHMS174_RS02345 475895..476248 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ECHMS174_RS02350 476290..477840 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECHMS174_RS02355 478004..478474 - 471 WP_000136192.1 YlaC family protein -
ECHMS174_RS02360 478590..479141 - 552 WP_000102564.1 maltose O-acetyltransferase -
ECHMS174_RS02365 479313..479531 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ECHMS174_RS02370 479557..479931 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECHMS174_RS02375 480477..483626 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
ECHMS174_RS02380 483649..484842 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T285392 WP_001291435.1 NZ_LM993812:c479531-479313 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT285392 WP_000344800.1 NZ_LM993812:c479931-479557 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References