Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 59269..59879 Replicon plasmid p67vangNB10
Accession NZ_LK021128
Organism Vibrio anguillarum strain NB10

Toxin (Protein)


Gene name PumA Uniprot ID A0A3M7LLW0
Locus tag VANGNB10_RS00265 Protein ID WP_038529385.1
Coordinates 59269..59565 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID Q6W4R6
Locus tag VANGNB10_RS00270 Protein ID WP_011154656.1
Coordinates 59589..59879 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VANGNB10_RS00245 54528..55442 - 915 WP_011154651.1 helicase RepA family protein -
VANGNB10_RS00250 55439..56464 - 1026 WP_020977927.1 hypothetical protein -
VANGNB10_RS00255 57384..58631 - 1248 WP_013856937.1 IS91-like element ISVa3 family transposase -
VANGNB10_RS00260 58910..59128 + 219 WP_107489848.1 hypothetical protein -
VANGNB10_RS00265 59269..59565 + 297 WP_038529385.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
VANGNB10_RS00270 59589..59879 + 291 WP_011154656.1 putative addiction module antidote protein Antitoxin
VANGNB10_RS20005 60008..61222 - 1215 WP_020977929.1 hypothetical protein -
VANGNB10_RS00285 61224..61607 - 384 WP_011154658.1 hypothetical protein -
VANGNB10_RS00290 61613..62584 - 972 WP_011154659.1 ParB/RepB/Spo0J family partition protein -
VANGNB10_RS00295 62581..63375 - 795 WP_020977930.1 ParA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - bauB 1..66798 66798


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(52-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11097.89 Da        Isoelectric Point: 10.7080

>T285380 WP_038529385.1 NZ_LK021128:59269-59565 [Vibrio anguillarum]
MFTIKRTQLFDDWLKTLKDAQARGAITARVQRLTQGLSGDVKPVGSGISELRIHVGKGYRVYFKRSGKQIVVLLCGGNKK
TQNQDIERAKELVQYFDL

Download         Length: 297 bp

>T285380 NZ_LK021128:59269-59565 [Vibrio anguillarum]
ATGTTCACTATAAAGCGCACGCAACTTTTTGATGATTGGTTGAAGACACTTAAAGATGCTCAGGCACGAGGTGCTATCAC
TGCTCGTGTTCAGCGCCTTACTCAGGGTTTGAGTGGGGATGTTAAGCCGGTTGGTTCGGGCATTAGTGAGCTTAGGATAC
ATGTCGGTAAAGGTTATCGAGTTTATTTTAAGCGTAGTGGAAAACAGATCGTTGTCTTACTTTGCGGTGGCAATAAGAAA
ACTCAGAATCAAGATATCGAACGCGCTAAAGAGTTGGTTCAATATTTTGATTTGTAG

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10273.59 Da        Isoelectric Point: 4.3148

>AT285380 WP_011154656.1 NZ_LK021128:59589-59879 [Vibrio anguillarum]
MNITRLDDFDPADYLDNAESIQAYIDEAVATGDAAFISQSLGVIARAKGMNTLAEETGLSRETLYRTLSVKGNPNLKTLI
SITNALGVSVKLGSYC

Download         Length: 291 bp

>AT285380 NZ_LK021128:59589-59879 [Vibrio anguillarum]
ATGAATATTACCAGGCTCGATGATTTTGATCCTGCTGACTATCTTGATAATGCTGAATCTATACAGGCATATATTGATGA
GGCGGTAGCAACTGGTGATGCTGCTTTTATTAGTCAATCCTTGGGTGTTATAGCCCGTGCCAAAGGTATGAATACGCTAG
CAGAGGAAACCGGTTTATCTCGTGAAACTTTATATAGAACATTAAGCGTTAAAGGGAATCCTAACCTTAAAACGCTTATC
TCTATCACTAATGCGCTTGGTGTATCTGTCAAACTCGGCTCTTACTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M7LLW0

Antitoxin

Source ID Structure
AlphaFold DB A0A3M7LK76

References