Detailed information of TA system TA028538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 7325520..7326103
• Replicon: chromosome
• Accession: NC_019729
• Organism: Oscillatoria nigro-viridis PCC 7112

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OSC7112_RS30355
• Protein ID: WP_041622810.1
• Coordinates: 7325756..7326103 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9VQT3
• Locus tag: OSC7112_RS30350
• Protein ID: WP_015179509.1
• Coordinates: 7325520..7325762 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OSC7112_RS30330	7320814..7321662	-	849	WP_015179504.1	alpha/beta hydrolase	-
OSC7112_RS30335	7321858..7322583	-	726	WP_015179505.1	DUF3120 domain-containing protein	-
OSC7112_RS30340	7322576..7323160	-	585	WP_015179506.1	hypothetical protein	-
OSC7112_RS30345	7323872..7325140	+	1269	WP_015179508.1	ferrochelatase	-
OSC7112_RS30350	7325520..7325762	+	243	WP_015179509.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
OSC7112_RS30355	7325756..7326103	+	348	WP_041622810.1	endoribonuclease MazF	Toxin
OSC7112_RS30360	7326123..7326395	+	273	WP_150111715.1	DUF433 domain-containing protein	-
OSC7112_RS30365	7326422..7326733	+	312	WP_015179512.1	DUF433 domain-containing protein	-
OSC7112_RS30370	7326751..7328007	-	1257	WP_015179513.1	aminotransferase class V-fold PLP-dependent enzyme	-
OSC7112_RS30375	7328103..7329923	+	1821	WP_015179514.1	TM0106 family RecB-like putative nuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (12-112)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12948.04 Da
• Isoelectric Point: 6.9777

>T28538 WP_041622810.1 NC_019729:7325756-7326103 [Oscillatoria nigro-viridis PCC 7112]
MVTQSNPYIPSRGDIVYLDFDPTKGHEQKGLRPAFVLSPRSYNEKSSLALFMPITKQQKGYPFEVILPTGLTVQGVILAD
QIKCLDWKARGIRLVESVSQDVIEEVQAKIEPLLF

Nucleotide

• Length: 348 bp

>T28538 NC_019729:7325756-7326103 [Oscillatoria nigro-viridis PCC 7112]
TTGGTGACACAATCAAATCCCTACATTCCGAGTCGAGGCGATATCGTTTATTTAGACTTTGACCCAACTAAGGGACACGA
GCAAAAAGGACTTAGACCTGCTTTTGTGCTGTCGCCTCGCTCCTACAACGAGAAAAGCTCCTTAGCTCTATTTATGCCGA
TTACAAAACAACAAAAAGGGTATCCTTTTGAAGTTATCCTACCCACTGGATTAACAGTTCAGGGCGTAATTCTTGCAGAT
CAAATTAAGTGTTTAGACTGGAAAGCTCGCGGTATTCGATTGGTTGAGTCGGTATCTCAAGATGTAATTGAAGAGGTGCA
AGCTAAAATAGAGCCGTTGTTATTCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8909.40 Da
• Isoelectric Point: 5.0659

>AT28538 WP_015179509.1 NC_019729:7325520-7325762 [Oscillatoria nigro-viridis PCC 7112]
MVGTIAKWGNSLAIRIPQNLAKKINLVEGSEVKLILIDGKLTIEPIVRRRYSLEELIEAMTPENLHTEIDTGVAVGNEVW

Nucleotide

• Length: 243 bp

>AT28538 NC_019729:7325520-7325762 [Oscillatoria nigro-viridis PCC 7112]
ATGGTTGGGACGATCGCAAAATGGGGAAACAGTCTTGCTATTCGGATTCCACAGAATTTAGCTAAAAAGATTAACTTGGT
TGAGGGTTCCGAAGTGAAGCTTATCTTGATAGATGGCAAGCTGACAATCGAGCCTATCGTTCGTCGGCGGTATTCGCTTG
AGGAGCTGATCGAAGCAATGACGCCAGAGAATCTTCATACTGAGATTGATACTGGAGTTGCTGTGGGGAACGAAGTTTGG
TGA

Structures

Antitoxin

• AlphaFold DB: K9VQT3