Detailed information of TA system TA028537

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 6566350..6567230
• Replicon: chromosome
• Accession: NC_019729
• Organism: Oscillatoria nigro-viridis PCC 7112

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: K9VP80
• Locus tag: OSC7112_RS27445
• Protein ID: WP_015178942.1
• Coordinates: 6566802..6567230 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: K9VR86
• Locus tag: OSC7112_RS27440
• Protein ID: WP_015178941.1
• Coordinates: 6566350..6566805 (+)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OSC7112_RS27415	6561674..6561973	-	300	WP_015178936.1	antibiotic biosynthesis monooxygenase	-
OSC7112_RS27420	6562047..6563984	+	1938	WP_015178937.1	2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase	-
OSC7112_RS27425	6564106..6564420	-	315	WP_015178938.1	glutaredoxin 3	-
OSC7112_RS27430	6564685..6564900	+	216	WP_015178939.1	hypothetical protein	-
OSC7112_RS27435	6565074..6565925	-	852	WP_015178940.1	WYL domain-containing protein	-
OSC7112_RS27440	6566350..6566805	+	456	WP_015178941.1	nucleotidyltransferase domain-containing protein	Antitoxin
OSC7112_RS27445	6566802..6567230	+	429	WP_015178942.1	DUF86 domain-containing protein	Toxin
OSC7112_RS27450	6567291..6570032	+	2742	WP_015178943.1	hypothetical protein	-
OSC7112_RS27455	6570044..6571060	+	1017	WP_015178944.1	type I-D CRISPR-associated protein Cas7/Csc2	-
OSC7112_RS27460	6571237..6572040	+	804	WP_015178945.1	type I-D CRISPR-associated protein Cas5/Csc1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (16-124)

Antitoxin

• Polymerase beta, nucleotidyltransferase (15-112)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16432.08 Da
• Isoelectric Point: 5.2523

>T28537 WP_015178942.1 NC_019729:6566802-6567230 [Oscillatoria nigro-viridis PCC 7112]
MSIQKTVVLTRLKFIGEYLLELRRFESMSLENYLASFDSKIISERIMELIVQAAVDINEHILTRGMKVNSDSNRESFVQL
GSYGIITPEVSEELVKSAGLRNILAHRYLEIDYVMLFNSVQVALIYYPIYIEQVAEYISQKK

Nucleotide

• Length: 429 bp

>T28537 NC_019729:6566802-6567230 [Oscillatoria nigro-viridis PCC 7112]
ATGAGTATTCAGAAAACAGTAGTTTTAACCAGACTCAAATTTATTGGAGAGTATCTGCTCGAATTGAGACGTTTTGAATC
GATGAGCCTGGAAAATTATCTAGCTAGCTTCGATTCAAAAATAATTTCAGAAAGAATAATGGAGTTGATAGTTCAGGCAG
CAGTTGATATTAACGAGCATATTCTGACCCGTGGAATGAAGGTTAACTCGGATAGTAATCGAGAATCTTTTGTTCAGCTT
GGAAGCTATGGAATTATTACACCTGAAGTTAGTGAAGAATTAGTAAAATCAGCAGGTTTGCGTAACATTTTGGCTCATCG
GTACTTAGAGATAGATTATGTAATGTTATTTAATTCCGTTCAAGTTGCTTTAATCTATTATCCGATTTACATAGAGCAAG
TTGCTGAATACATTTCTCAAAAAAAATGA

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 17535.79 Da
• Isoelectric Point: 4.2036

>AT28537 WP_015178941.1 NC_019729:6566350-6566805 [Oscillatoria nigro-viridis PCC 7112]
MLQISPTLTELQEIASQLPEQIPYLKMLILFGSRARGDTHAKSDWDFAALYDEKLREESCNNRGFAWFEVPGILGEAFSI
NSDEIDVVELNRCSPLIANFIARDGKLIYEQESGQFEQFVKTHLMNELELQELERQLYQSIDDFLKDWRSV

Nucleotide

• Length: 456 bp

>AT28537 NC_019729:6566350-6566805 [Oscillatoria nigro-viridis PCC 7112]
ATGCTCCAAATTTCCCCAACGCTGACTGAACTTCAGGAAATTGCTTCACAACTTCCCGAACAAATTCCTTACCTCAAAAT
GCTAATTCTCTTTGGTTCTCGTGCCAGAGGTGATACTCACGCAAAAAGTGATTGGGATTTTGCCGCACTTTACGATGAAA
AGCTTAGGGAAGAATCTTGCAATAATCGAGGCTTTGCATGGTTTGAAGTTCCTGGCATTTTGGGTGAAGCTTTTAGCATT
AACAGCGATGAAATTGATGTCGTTGAATTGAATCGCTGTTCCCCTCTGATTGCCAATTTTATTGCTCGTGATGGCAAACT
TATTTATGAACAAGAATCAGGTCAGTTTGAGCAATTTGTCAAAACTCATCTGATGAACGAGCTGGAATTGCAAGAACTGG
AACGTCAACTTTATCAGAGTATTGATGATTTTCTCAAAGATTGGAGGTCGGTATGA

Structures

Toxin

• AlphaFold DB: K9VP80

Antitoxin

• AlphaFold DB: K9VR86