Detailed information of TA system TA028536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/DUF1778(antitoxin)
• Location: 5116658..5117466
• Replicon: chromosome
• Accession: NC_019729
• Organism: Oscillatoria nigro-viridis PCC 7112

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: K9VN34
• Locus tag: OSC7112_RS21420
• Protein ID: WP_015177863.1
• Coordinates: 5116945..5117466 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: K9VM60
• Locus tag: OSC7112_RS21415
• Protein ID: WP_015177862.1
• Coordinates: 5116658..5116954 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OSC7112_RS21395	5112240..5113292	+	1053	WP_041623342.1	putative DNA binding domain-containing protein	-
OSC7112_RS21400	5113379..5113645	-	267	WP_006631847.1	hypothetical protein	-
OSC7112_RS21405	5113693..5114769	-	1077	WP_041622671.1	branched-chain amino acid ABC transporter permease	-
OSC7112_RS21410	5114970..5116364	-	1395	WP_015177861.1	branched-chain amino acid ABC transporter substrate-binding protein	-
OSC7112_RS21415	5116658..5116954	+	297	WP_015177862.1	DUF1778 domain-containing protein	Antitoxin
OSC7112_RS21420	5116945..5117466	+	522	WP_015177863.1	GNAT family N-acetyltransferase	Toxin
OSC7112_RS21425	5117570..5118517	+	948	WP_015177864.1	NAD(P)-dependent oxidoreductase	-
OSC7112_RS21430	5118577..5118987	+	411	WP_015177865.1	hypothetical protein	-
OSC7112_RS21435	5118984..5119865	+	882	WP_015177866.1	RNA polymerase sigma-70 factor	-
OSC7112_RS21440	5120016..5120297	+	282	WP_015177867.1	hexameric tyrosine-coordinated heme protein	-
OSC7112_RS21445	5120359..5121039	+	681	WP_041623345.1	plastoquinol terminal oxidase	-
OSC7112_RS21450	5121127..5121504	+	378	WP_015177869.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (52-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (18-92)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19064.08 Da
• Isoelectric Point: 7.6686

>T28536 WP_015177863.1 NC_019729:5116945-5117466 [Oscillatoria nigro-viridis PCC 7112]
VGLDRDLDQLCPPEKLNSLHQIETFDSGNSQLDEWLKRRALKNESEGASRTYVLCDRQAVIAYYCLANGAIAKTAATGKV
RRNMPDPIPVMVIGRLAVDRDWQGLGIGRALLRDAILRTLQAAEIAGIRAILVHAISEDAKQFYQKCGFTVSPIDPMTLM
VKISDAIASLELK

Nucleotide

• Length: 522 bp

>T28536 NC_019729:5116945-5117466 [Oscillatoria nigro-viridis PCC 7112]
GTGGGATTAGATCGAGATTTAGACCAACTCTGCCCGCCGGAAAAACTGAACTCGTTACATCAAATTGAGACCTTTGATTC
AGGCAACAGTCAGTTAGATGAATGGCTCAAGCGCCGCGCTCTGAAAAATGAGTCAGAGGGCGCTTCACGGACTTATGTTT
TGTGCGATCGTCAAGCAGTCATTGCGTACTATTGTCTTGCAAATGGAGCAATAGCAAAAACTGCTGCAACGGGTAAAGTC
CGGCGCAATATGCCCGATCCGATTCCTGTTATGGTGATTGGACGGCTTGCAGTCGATCGGGATTGGCAAGGCCTCGGCAT
CGGACGCGCTTTGCTGCGGGATGCAATTCTTCGCACTTTGCAAGCTGCTGAGATTGCAGGGATACGGGCAATTCTCGTAC
ACGCTATTTCGGAAGATGCGAAGCAGTTTTATCAGAAGTGCGGGTTTACTGTTTCGCCGATCGATCCAATGACACTCATG
GTGAAAATTAGCGATGCCATTGCTTCACTTGAGCTAAAGTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11099.70 Da
• Isoelectric Point: 9.0774

>AT28536 WP_015177862.1 NC_019729:5116658-5116954 [Oscillatoria nigro-viridis PCC 7112]
MHPPASEESASQTRDVTINIRAKPNQRALIDRAAQLQGKSRSEFMLESAYQKAQDVLLDRCFFGLDEIKFKQFLALLDAP
PTPNQKLNALLKTKSPWD

Nucleotide

• Length: 297 bp

>AT28536 NC_019729:5116658-5116954 [Oscillatoria nigro-viridis PCC 7112]
ATGCACCCGCCAGCATCTGAAGAATCAGCCAGCCAGACCCGTGATGTGACAATCAACATTCGAGCTAAGCCGAATCAACG
CGCTCTCATCGATCGCGCGGCCCAATTACAGGGAAAGAGCCGATCGGAGTTCATGCTTGAATCAGCTTACCAAAAAGCTC
AAGATGTTCTTCTAGACCGCTGTTTTTTTGGGTTAGATGAAATTAAATTTAAGCAGTTTTTGGCGCTGCTGGATGCACCG
CCGACGCCAAATCAGAAGCTAAACGCATTACTAAAAACAAAATCTCCGTGGGATTAG

Structures

Toxin

• AlphaFold DB: K9VN34

Antitoxin

• AlphaFold DB: K9VM60