Detailed information of TA system TA285359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/-
• Location: 373429..374080
• Replicon: chromosome
• Accession: NZ_JMSI01000003
• Organism: Lacticaseibacillus rhamnosus 51B

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: LR51B_RS06400
• Protein ID: WP_005686631.1
• Coordinates: 373697..374080 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: LR51B_RS06405
• Protein ID: WP_005686632.1
• Coordinates: 373429..373677 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LR51B_RS06425 (LR51B_07450)	368478..369866	+	1389	WP_014571607.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
LR51B_RS06420 (LR51B_07455)	370140..371648	+	1509	WP_005686635.1	DEAD/DEAH box helicase	-
LR51B_RS06415 (LR51B_07460)	371817..372191	+	375	WP_005686634.1	holo-ACP synthase	-
LR51B_RS06410 (LR51B_07465)	372178..373317	+	1140	WP_005691215.1	alanine racemase	-
LR51B_RS06405 (LR51B_07470)	373429..373677	+	249	WP_005686632.1	antitoxin	Antitoxin
LR51B_RS06400 (LR51B_07475)	373697..374080	+	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LR51B_RS06395 (LR51B_07490)	374594..375121	+	528	WP_032956972.1	QueT transporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T285359 WP_005686631.1 NZ_JMSI01000003:373697-374080 [Lacticaseibacillus rhamnosus 51B]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T285359 NZ_JMSI01000003:373697-374080 [Lacticaseibacillus rhamnosus 51B]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAACAATGTTGGTAACCATTATAGCCCCACTGTTATCGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACCCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT285359 WP_005686632.1 NZ_JMSI01000003:373429-373677 [Lacticaseibacillus rhamnosus 51B]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT285359 NZ_JMSI01000003:373429-373677 [Lacticaseibacillus rhamnosus 51B]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAATTGATTT
CTGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19