Detailed information of TA system TA028534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-chpS/PRK09907-MazE
• Location: 806717..807315
• Replicon: chromosome
• Accession: NC_019729
• Organism: Oscillatoria nigro-viridis PCC 7112

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K9VCT9
• Locus tag: OSC7112_RS03560
• Protein ID: WP_015174617.1
• Coordinates: 806959..807315 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: K9VBM9
• Locus tag: OSC7112_RS03555
• Protein ID: WP_015174616.1
• Coordinates: 806717..806965 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OSC7112_RS03525	802547..803767	-	1221	WP_015174611.1	(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
OSC7112_RS03530	804062..805441	+	1380	WP_015174612.1	GUN4 domain-containing protein	-
OSC7112_RS03535	805492..805902	+	411	WP_015174613.1	hypothetical protein	-
OSC7112_RS03540	805902..806144	+	243	Protein_679	hypothetical protein	-
OSC7112_RS03545	806178..806384	+	207	WP_041622897.1	type II toxin-antitoxin system HicB family antitoxin	-
OSC7112_RS03550	806371..806595	+	225	WP_015174615.1	hypothetical protein	-
OSC7112_RS03555	806717..806965	+	249	WP_015174616.1	SpoVT/AbrB domain-containing protein	Antitoxin
OSC7112_RS03560	806959..807315	+	357	WP_015174617.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OSC7112_RS03565	807467..807763	+	297	WP_015174618.1	hypothetical protein	-
OSC7112_RS03570	807750..807974	+	225	WP_015174619.1	hypothetical protein	-
OSC7112_RS03575	808216..808767	+	552	WP_015174620.1	Uma2 family endonuclease	-
OSC7112_RS03580	808896..809846	-	951	WP_015174622.1	Rpn family recombination-promoting nuclease/putative transposase	-
OSC7112_RS03585	809985..810350	-	366	WP_015174623.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-109)

Antitoxin

• Antitoxin PemI-like (4-82)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13637.71 Da
• Isoelectric Point: 7.1345

>T28534 WP_015174617.1 NC_019729:806959-807315 [Oscillatoria nigro-viridis PCC 7112]
MVNYIPERGHFIRLNFDPQSGHEQMGTRPALVVSQTKFNRKMGFVFVCPISNTQRKNPFYVPIPEGETITGVIMTDQLRS
LDYSARQASFLSECRADIIEEVLRRIQPILFEKMTVDE

Nucleotide

• Length: 357 bp

>T28534 NC_019729:806959-807315 [Oscillatoria nigro-viridis PCC 7112]
TTGGTAAACTATATCCCCGAACGAGGTCATTTTATTCGCCTCAATTTTGACCCTCAATCAGGACACGAACAAATGGGGAC
TAGGCCTGCACTGGTGGTAAGTCAAACTAAATTTAACCGAAAAATGGGGTTTGTGTTTGTTTGTCCCATCAGCAACACAC
AACGCAAAAATCCGTTTTATGTTCCCATTCCCGAAGGTGAAACGATAACAGGTGTAATTATGACGGATCAACTCCGCTCT
TTAGATTATAGCGCAAGGCAGGCAAGTTTTCTTAGTGAATGTCGAGCAGATATAATTGAGGAAGTTTTGAGGCGAATTCA
ACCTATTTTGTTTGAGAAGATGACTGTTGATGAATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9085.43 Da
• Isoelectric Point: 4.3351

>AT28534 WP_015174616.1 NC_019729:806717-806965 [Oscillatoria nigro-viridis PCC 7112]
MTAKIHISKLGNSLALPIPDYIVKFLNLKAEDSVICSIENGKLVIEPVNNKISKYTLDELLAGEIESSEEVSWGKPEGEE
VW

Nucleotide

• Length: 249 bp

>AT28534 NC_019729:806717-806965 [Oscillatoria nigro-viridis PCC 7112]
ATGACAGCCAAAATACACATCAGCAAATTAGGTAATTCCTTGGCTTTGCCAATACCAGACTACATAGTAAAGTTTCTCAA
CCTTAAAGCCGAAGATTCAGTAATTTGCAGCATAGAAAATGGGAAACTGGTCATAGAGCCTGTAAATAATAAAATATCAA
AGTATACCCTAGATGAATTATTGGCAGGCGAAATAGAATCTAGCGAAGAAGTCTCTTGGGGAAAACCAGAAGGAGAGGAA
GTTTGGTAA

Structures

Toxin

• AlphaFold DB: K9VCT9

Antitoxin

• AlphaFold DB: K9VBM9