285325

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 3157311..3159031 Replicon chromosome
Accession NZ_HG994851
Organism Escherichia coli isolate 127

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag LLO82_RS15235 Protein ID WP_000312833.1
Coordinates 3157311..3157967 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag LLO82_RS15240 Protein ID WP_000634203.1
Coordinates 3157964..3159031 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLO82_RS15215 (3152711) 3152711..3152965 - 255 WP_154670580.1 hypothetical protein -
LLO82_RS15220 (3153247) 3153247..3153486 + 240 WP_001431807.1 hypothetical protein -
LLO82_RS15225 (3153526) 3153526..3155964 + 2439 WP_000985465.1 type I restriction-modification system subunit M -
LLO82_RS15230 (3155964) 3155964..3157298 + 1335 WP_000228013.1 restriction endonuclease subunit S -
LLO82_RS15235 (3157311) 3157311..3157967 + 657 WP_000312833.1 toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT Toxin
LLO82_RS15240 (3157964) 3157964..3159031 + 1068 WP_000634203.1 toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG Antitoxin
LLO82_RS15245 (3159050) 3159050..3162145 + 3096 WP_000108735.1 HsdR family type I site-specific deoxyribonuclease -
LLO82_RS15250 (3162145) 3162145..3162861 + 717 WP_001122107.1 SprT family zinc-dependent metalloprotease -
LLO82_RS15255 (3163206) 3163206..3163820 + 615 Protein_2993 IS3-like element IS600 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - aafC / aggD / iroC / iroD / iroE / iroN 3140154..3188387 48233


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T285325 WP_000312833.1 NZ_HG994851:3157311-3157967 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT285325 WP_000634203.1 NZ_HG994851:3157964-3159031 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References