Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2531728..2533316 Replicon chromosome
Accession NZ_HG994850
Organism Escherichia coli isolate 127

Toxin (Protein)


Gene name hipA Uniprot ID A0A192ENQ5
Locus tag LLO83_RS12010 Protein ID WP_001125432.1
Coordinates 2531994..2533316 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag LLO83_RS12005 Protein ID WP_001304368.1
Coordinates 2531728..2531994 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLO83_RS11990 (2528096) 2528096..2529547 + 1452 WP_000854642.1 tagaturonate reductase -
LLO83_RS11995 (2529744) 2529744..2530658 + 915 WP_001298637.1 bestrophin family protein -
LLO83_RS12000 (2530662) 2530662..2531420 - 759 WP_001286538.1 trans-aconitate 2-methyltransferase -
LLO83_RS12005 (2531728) 2531728..2531994 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
LLO83_RS12010 (2531994) 2531994..2533316 + 1323 WP_001125432.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
LLO83_RS12015 (2533492) 2533492..2534184 - 693 WP_000382466.1 hypothetical protein -
LLO83_RS12020 (2534196) 2534196..2534471 - 276 WP_000948310.1 hypothetical protein -
LLO83_RS12025 (2534473) 2534473..2534613 - 141 WP_001335097.1 hypothetical protein -
LLO83_RS12030 (2534729) 2534729..2534911 - 183 WP_001335098.1 hypothetical protein -
LLO83_RS12035 (2535081) 2535081..2536133 + 1053 WP_000568119.1 phage/plasmid replication protein, II/X family -
LLO83_RS12040 (2536145) 2536145..2536462 + 318 WP_000073834.1 DNA-binding protein -
LLO83_RS12050 (2536666) 2536666..2536908 + 243 WP_000699011.1 phage capsid protein -
LLO83_RS12055 (2536955) 2536955..2538235 + 1281 WP_000823306.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49297.02 Da        Isoelectric Point: 8.6972

>T285294 WP_001125432.1 NZ_HG994850:2531994-2533316 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT285294 WP_001304368.1 NZ_HG994850:2531728-2531994 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192ENQ5

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References