Detailed information of TA system TA028529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 459570..460144
• Replicon: chromosome
• Accession: NC_019702
• Organism: Synechococcus sp. PCC 7502

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K9SR24
• Locus tag: SYN7502_RS02195
• Protein ID: WP_015167264.1
• Coordinates: 459570..459905 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9SRK3
• Locus tag: SYN7502_RS02200
• Protein ID: WP_015167265.1
• Coordinates: 459902..460144 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SYN7502_RS18055	454665..455273	+	609	WP_015167257.1	type II secretion system GspH family protein	-
SYN7502_RS02165	455283..455516	+	234	WP_041429179.1	hypothetical protein	-
SYN7502_RS02170	455558..456586	+	1029	WP_015167259.1	ABC transporter permease	-
SYN7502_RS02175	456552..457301	-	750	WP_144050157.1	DUF502 domain-containing protein	-
SYN7502_RS02180	457537..457977	+	441	WP_015167261.1	tRNA (cytidine(34)-2'-O)-methyltransferase	-
SYN7502_RS02185	457985..458812	-	828	WP_015167262.1	hypothetical protein	-
SYN7502_RS02190	458855..459532	-	678	WP_015167263.1	hypothetical protein	-
SYN7502_RS02195	459570..459905	-	336	WP_015167264.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SYN7502_RS02200	459902..460144	-	243	WP_015167265.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SYN7502_RS02205	460252..461724	+	1473	WP_015167266.1	D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase	-
SYN7502_RS02210	461838..462161	-	324	WP_015167267.1	DUF3067 family protein	-
SYN7502_RS02215	462328..463044	+	717	WP_015167268.1	ROK family protein	-
SYN7502_RS02220	463130..463651	+	522	WP_015167269.1	DUF4214 domain-containing protein	-
SYN7502_RS02225	463684..464499	+	816	WP_015167270.1	tetratricopeptide repeat protein	-
SYN7502_RS02230	464638..464988	+	351	WP_015167271.1	STAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-107)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12353.48 Da
• Isoelectric Point: 5.1102

>T28529 WP_015167264.1 NC_019702:c459905-459570 [Synechococcus sp. PCC 7502]
MSSYIPERGDILKINFSPQIGSEQAGFRPALVISPSSYNRLSSLAIICPITSKQKGLNFEVLLPEGLQTYGVVLCDQIKS
LDWKARGAFLIERISMELMEEVLARIEPLVL

Nucleotide

• Length: 336 bp

>T28529 NC_019702:c459905-459570 [Synechococcus sp. PCC 7502]
TTGAGCAGCTATATACCTGAACGTGGCGATATTCTCAAAATCAACTTTTCTCCTCAGATAGGTTCAGAGCAAGCTGGATT
CAGACCTGCCTTAGTAATTTCTCCAAGTTCATATAACCGACTATCTTCATTAGCAATTATTTGTCCGATAACCAGCAAGC
AAAAGGGTTTGAATTTTGAGGTGTTATTACCTGAAGGCTTACAAACCTACGGCGTGGTTCTTTGCGATCAAATCAAGAGC
CTTGACTGGAAAGCTAGAGGAGCTTTTTTGATAGAGCGAATATCGATGGAATTAATGGAAGAAGTATTAGCAAGGATAGA
GCCATTAGTTTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8908.30 Da
• Isoelectric Point: 7.2774

>AT28529 WP_015167265.1 NC_019702:c460144-459902 [Synechococcus sp. PCC 7502]
MKATISKWGNSLAFRIPKHIAESLQLQEGSSVSISVSDNCLTIAPLKRKKYTLEELLKDMTPEHFHLEISTGNAIGQEIL

Nucleotide

• Length: 243 bp

>AT28529 NC_019702:c460144-459902 [Synechococcus sp. PCC 7502]
ATGAAAGCCACAATTTCCAAATGGGGAAACAGTCTTGCCTTTCGCATACCTAAACATATAGCTGAGAGCCTTCAACTCCA
AGAAGGAAGCTCTGTAAGTATCTCCGTATCAGATAATTGCTTGACGATCGCTCCATTGAAACGCAAAAAATATACCCTAG
AGGAACTATTGAAAGATATGACTCCTGAGCATTTTCATCTAGAAATTTCCACAGGCAATGCCATTGGGCAAGAAATCCTT
TGA

Structures

Toxin

• AlphaFold DB: K9SR24

Antitoxin

• AlphaFold DB: K9SRK3