Detailed information of TA system TA028528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 130138..130627
• Replicon: chromosome
• Accession: NC_019701
• Organism: Pseudanabaena sp. PCC 7367

Toxin (Protein)

• Gene name: -
• Uniprot ID: K9SEN0
• Locus tag: PSE7367_RS00505
• Protein ID: WP_015163395.1
• Coordinates: 130138..130407 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: K9SCT2
• Locus tag: PSE7367_RS00510
• Protein ID: WP_015163396.1
• Coordinates: 130388..130627 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSE7367_RS00480	125701..126723	+	1023	WP_198013459.1	CIA30 family protein	-
PSE7367_RS00485	126877..127842	+	966	WP_015163391.1	tetratricopeptide repeat protein	-
PSE7367_RS00490	128042..128410	-	369	WP_015163392.1	cyclic nucleotide-binding domain-containing protein	-
PSE7367_RS00495	128583..129002	-	420	WP_015163393.1	peptidylprolyl isomerase	-
PSE7367_RS00500	129218..129898	-	681	WP_015163394.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
PSE7367_RS00505	130138..130407	+	270	WP_015163395.1	BrnT family toxin	Toxin
PSE7367_RS00510	130388..130627	+	240	WP_015163396.1	hypothetical protein	Antitoxin
PSE7367_RS00515	130649..131179	-	531	WP_015163397.1	GIY-YIG nuclease family protein	-
PSE7367_RS00520	131315..131533	-	219	WP_015163398.1	DUF2839 domain-containing protein	-
PSE7367_RS00525	131705..132550	-	846	WP_015163399.1	hypothetical protein	-
PSE7367_RS00530	132630..133127	-	498	WP_041698256.1	TIGR02652 family protein	-
PSE7367_RS00535	133446..134456	-	1011	WP_015163401.1	phosphoribulokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10784.10 Da
• Isoelectric Point: 5.6802

>T28528 WP_015163395.1 NC_019701:130138-130407 [Pseudanabaena sp. PCC 7367]
MEFEFDDRKNQTNQAKHGIDFHEAQLLWDDCDRIEIPARTEDEPRYLVIARLNAKHYSAVITYRDDKVRIISVRRARVEE
IEIYESKRF

Nucleotide

• Length: 270 bp

>T28528 NC_019701:130138-130407 [Pseudanabaena sp. PCC 7367]
ATGGAATTCGAGTTTGACGATCGCAAAAACCAAACTAACCAAGCAAAACACGGGATCGACTTTCATGAAGCTCAATTACT
CTGGGACGATTGCGATCGGATCGAAATTCCCGCCCGCACCGAGGATGAGCCGCGCTATTTGGTAATCGCTCGATTGAATG
CCAAGCATTACTCCGCCGTGATTACCTATCGAGACGACAAAGTCCGCATTATTTCAGTTCGCCGCGCCCGCGTGGAAGAG
ATAGAAATTTATGAAAGCAAAAGATTTTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9233.50 Da
• Isoelectric Point: 8.6589

>AT28528 WP_015163396.1 NC_019701:130388-130627 [Pseudanabaena sp. PCC 7367]
MKAKDFDQKFNNGEDITAFLDLSKARRPNHDQRRVNVDFPIWMIAALDYEANRLGVTRQSIIKVWIAERLEQTARAELS

Nucleotide

• Length: 240 bp

>AT28528 NC_019701:130388-130627 [Pseudanabaena sp. PCC 7367]
ATGAAAGCAAAAGATTTTGATCAGAAATTCAACAACGGGGAAGACATCACGGCATTCCTCGATCTTTCAAAAGCTCGCCG
TCCCAACCATGATCAGCGGCGGGTTAACGTAGATTTTCCAATTTGGATGATCGCAGCGCTCGACTATGAAGCAAATCGCC
TTGGTGTGACCCGCCAATCGATCATCAAGGTATGGATCGCCGAACGATTGGAGCAAACTGCCAGGGCTGAATTATCCTGA

Structures

Toxin

• AlphaFold DB: K9SEN0

Antitoxin

• AlphaFold DB: K9SCT2