Detailed information of TA system TA028524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3779273..3779835
• Replicon: chromosome
• Accession: NC_019695
• Organism: Chroococcidiopsis thermalis PCC 7203

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K9U349
• Locus tag: CHRO_RS16580
• Protein ID: WP_015155386.1
• Coordinates: 3779503..3779835 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9U1Z6
• Locus tag: CHRO_RS16575
• Protein ID: WP_015155385.1
• Coordinates: 3779273..3779509 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHRO_RS16530	3774446..3774637	+	192	WP_015155377.1	hypothetical protein	-
CHRO_RS16535	3774637..3775572	+	936	WP_181824373.1	cobalt-precorrin-6A reductase	-
CHRO_RS16545	3776104..3776712	-	609	WP_041463216.1	2OG-Fe(II) oxygenase	-
CHRO_RS16550	3776812..3777237	-	426	WP_015155381.1	PIN domain-containing protein	-
CHRO_RS16555	3777234..3777494	-	261	WP_015155382.1	hypothetical protein	-
CHRO_RS29725	3777782..3777961	+	180	WP_051033245.1	hypothetical protein	-
CHRO_RS16565	3778115..3778309	+	195	WP_015155383.1	heavy-metal-associated domain-containing protein	-
CHRO_RS16570	3778287..3779192	-	906	WP_015155384.1	alpha/beta hydrolase	-
CHRO_RS16575	3779273..3779509	+	237	WP_015155385.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CHRO_RS16580	3779503..3779835	+	333	WP_015155386.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CHRO_RS16585	3779893..3780105	+	213	WP_015155387.1	type II toxin-antitoxin system VapB family antitoxin	-
CHRO_RS16590	3780092..3780493	+	402	WP_015155388.1	PIN domain nuclease	-
CHRO_RS16595	3780562..3781536	+	975	WP_015155389.1	tyrosine-type recombinase/integrase	-
CHRO_RS16600	3781612..3782763	-	1152	WP_015155390.1	tyrosine-type recombinase/integrase	-
CHRO_RS16605	3783162..3783746	+	585	WP_015155391.1	DUF99 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-108)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12562.72 Da
• Isoelectric Point: 9.3549

>T28524 WP_015155386.1 NC_019695:3779503-3779835 [Chroococcidiopsis thermalis PCC 7203]
VVEVPARGTFIWLNFEPQSGREQMGRRPALVVSHTAFNRKRGFAFVCPISNTRRKNPFYIAIPEGLAVTGVIMCDQLRSL
DYRIRNAEFLGECPISLLEEVLLRIQPIFL

Nucleotide

• Length: 333 bp

>T28524 NC_019695:3779503-3779835 [Chroococcidiopsis thermalis PCC 7203]
GTGGTAGAAGTTCCAGCGCGAGGTACTTTCATTTGGCTGAATTTTGAACCGCAATCTGGACGCGAACAAATGGGTAGAAG
ACCTGCTTTAGTTGTTAGCCATACAGCTTTTAACCGCAAGCGTGGTTTTGCGTTCGTTTGTCCCATCAGTAATACTAGGC
GGAAAAATCCTTTTTATATTGCGATTCCAGAAGGTTTAGCTGTAACTGGAGTCATCATGTGCGACCAATTGCGATCGCTA
GATTACCGCATCAGAAATGCAGAGTTTTTAGGTGAATGTCCTATTTCCCTACTAGAAGAAGTCTTACTCCGAATTCAACC
GATCTTCTTGTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9121.44 Da
• Isoelectric Point: 4.9155

>AT28524 WP_015155385.1 NC_019695:3779273-3779509 [Chroococcidiopsis thermalis PCC 7203]
MRSSIGKWGNSLGLRIPRHIVEELSLHPNDEVECRVEKGRLIVEVVHKRKYTLEELLSQEMEPEPEIDWGKPMGEEAW

Nucleotide

• Length: 237 bp

>AT28524 NC_019695:3779273-3779509 [Chroococcidiopsis thermalis PCC 7203]
ATGCGAAGTTCAATCGGTAAATGGGGAAATTCGCTGGGATTGAGGATACCCAGACATATTGTAGAAGAATTATCTCTGCA
TCCAAATGATGAAGTTGAGTGTCGCGTTGAAAAAGGTAGATTGATTGTTGAGGTCGTACACAAGCGCAAATACACCTTAG
AGGAATTGCTTTCTCAAGAGATGGAACCCGAACCAGAAATCGACTGGGGTAAACCTATGGGTGAAGAAGCGTGGTAG

Structures

Toxin

• AlphaFold DB: K9U349

Antitoxin

• AlphaFold DB: K9U1Z6