Detailed information of TA system TA028522

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 7491473..7492371
• Replicon: chromosome
• Accession: NC_019693
• Organism: Oscillatoria acuminata PCC 6304

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: OSCIL6304_RS28905
• Protein ID: WP_015151917.1
• Coordinates: 7491916..7492371 (+)
• Length: 152 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: K9TT31
• Locus tag: OSCIL6304_RS28900
• Protein ID: WP_015151916.1
• Coordinates: 7491473..7491919 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OSCIL6304_RS28885	7487626..7488804	+	1179	WP_015151913.1	response regulator	-
OSCIL6304_RS28890	7488882..7489337	+	456	WP_044198197.1	response regulator	-
OSCIL6304_RS28895	7489522..7490997	-	1476	WP_015151915.1	glycogen synthase GlgA	-
OSCIL6304_RS28900	7491473..7491919	+	447	WP_015151916.1	nucleotidyltransferase domain-containing protein	Antitoxin
OSCIL6304_RS28905	7491916..7492371	+	456	WP_015151917.1	DUF86 domain-containing protein	Toxin
OSCIL6304_RS28910	7492476..7493342	-	867	WP_015151918.1	WYL domain-containing protein	-
OSCIL6304_RS28915	7493417..7496137	+	2721	WP_015151919.1	hypothetical protein	-
OSCIL6304_RS28920	7496149..7497174	+	1026	WP_015151920.1	type I-D CRISPR-associated protein Cas7/Csc2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (16-133)

Antitoxin

• Polymerase beta, nucleotidyltransferase (21-110)

Sequences

Toxin

Protein

• Length: 152 a.a.
• Molecular weight: 17099.46 Da
• Isoelectric Point: 4.4273

>T28522 WP_015151917.1 NC_019693:7491916-7492371 [Oscillatoria acuminata PCC 6304]
MSSIEPVVVSSRIRLISEYLEILQDFEGMELGEYLANIRHQLLAERILEILIQAAIDINRHILKSGQSLESLRNEETFLQ
MGNSGVISEKLAENLARSGGFRNVLAHHYLKIDSRLVFASIQDTLEFYPLYVQEIVTYLESLEESIGDSSG

Nucleotide

• Length: 456 bp

>T28522 NC_019693:7491916-7492371 [Oscillatoria acuminata PCC 6304]
ATGAGTTCTATCGAGCCAGTCGTGGTCAGTAGCCGAATTCGTTTGATTTCTGAATATCTGGAAATCCTCCAAGATTTTGA
AGGGATGGAACTCGGTGAATATTTGGCAAATATTCGTCATCAATTATTGGCAGAGAGAATTTTGGAAATCCTGATTCAGG
CTGCAATTGACATCAATCGACATATTTTAAAATCAGGGCAATCCCTGGAAAGCCTCAGAAATGAAGAAACGTTTTTACAG
ATGGGTAACAGCGGGGTGATTTCCGAAAAATTAGCCGAAAACCTCGCCAGATCCGGCGGCTTTCGCAACGTATTAGCGCA
TCACTATCTCAAAATTGATAGTCGCCTCGTTTTTGCATCGATTCAAGATACTTTAGAATTTTATCCGCTTTATGTCCAGG
AAATTGTCACTTATTTAGAATCTTTAGAGGAAAGCATCGGTGACAGTTCAGGATAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16997.29 Da
• Isoelectric Point: 6.3794

>AT28522 WP_015151916.1 NC_019693:7491473-7491919 [Oscillatoria acuminata PCC 6304]
MNELPLTELSTVAPQLHGKIPELKLLILFGSRARGDFQAQSDWDFAVLSDEKLNENSLKNNFHLTLETSNFLSELLGISS
DKLDIVQLNRAGALISHFVARDGKLVYERETGEFERFCHRALLEKSDLQKIHHENLTTVHQFLNRWRA

Nucleotide

• Length: 447 bp

>AT28522 NC_019693:7491473-7491919 [Oscillatoria acuminata PCC 6304]
ATGAATGAACTACCCCTCACCGAACTCTCAACCGTTGCTCCACAACTGCACGGCAAGATTCCTGAACTCAAACTGCTGAT
TTTATTTGGATCAAGAGCTAGAGGAGATTTTCAAGCTCAGAGTGACTGGGACTTTGCTGTTTTATCTGATGAAAAATTGA
ATGAAAATAGTTTGAAAAATAACTTTCATTTAACCCTTGAAACCTCTAATTTTCTCAGTGAATTATTGGGGATTTCTAGC
GATAAACTAGATATCGTCCAACTCAATCGAGCTGGGGCGCTGATCTCTCATTTTGTGGCTCGGGATGGAAAGCTAGTATA
TGAGCGGGAGACGGGAGAGTTTGAGCGGTTTTGCCATCGAGCTTTACTTGAAAAATCAGACCTTCAAAAAATTCATCATG
AGAATTTAACAACGGTTCATCAGTTTTTGAACAGGTGGAGAGCATGA

Structures

Antitoxin

• AlphaFold DB: K9TT31