285212

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5055662..5056238 Replicon chromosome
Accession NZ_HG941718
Organism Escherichia coli O25b:H4-ST131 strain EC958

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag EC958_RS25275 Protein ID WP_001295743.1
Coordinates 5055662..5055949 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag EC958_RS25280 Protein ID WP_000063148.1
Coordinates 5055936..5056238 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC958_RS25260 5051322..5052626 - 1305 WP_000535012.1 restriction endonuclease subunit S -
EC958_RS25265 5052616..5054235 - 1620 WP_001029745.1 type I restriction-modification system subunit M -
EC958_RS25270 5054299..5055465 - 1167 WP_000800831.1 restriction endonuclease -
EC958_RS25275 5055662..5055949 + 288 WP_001295743.1 BrnT family toxin Toxin
EC958_RS25280 5055936..5056238 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
EC958_RS25285 5056272..5057228 - 957 WP_001295745.1 GTPase -
EC958_RS25290 5057239..5057442 - 204 WP_000467859.1 YbdD/YjiX family protein -
EC958_RS25295 5057492..5059642 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T285212 WP_001295743.1 NZ_HG941718:5055662-5055949 [Escherichia coli O25b:H4-ST131]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT285212 WP_000063148.1 NZ_HG941718:5055936-5056238 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References