Detailed information of TA system TA028521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/GNAT-DUF1778
• Location: 5890716..5891521
• Replicon: chromosome
• Accession: NC_019693
• Organism: Oscillatoria acuminata PCC 6304

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: K9TPL3
• Locus tag: OSCIL6304_RS22685
• Protein ID: WP_015150731.1
• Coordinates: 5890716..5891234 (-)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: K9TML6
• Locus tag: OSCIL6304_RS22690
• Protein ID: WP_015150732.1
• Coordinates: 5891225..5891521 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OSCIL6304_RS22665	5886642..5887016	-	375	WP_015150728.1	hypothetical protein	-
OSCIL6304_RS22670	5887277..5888146	-	870	WP_015150729.1	Uma2 family endonuclease	-
OSCIL6304_RS22675	5888374..5889456	+	1083	WP_015149763.1	photosystem II q(b) protein	-
OSCIL6304_RS22680	5889902..5890360	-	459	WP_015150730.1	hypothetical protein	-
OSCIL6304_RS22685	5890716..5891234	-	519	WP_015150731.1	GNAT family N-acetyltransferase	Toxin
OSCIL6304_RS22690	5891225..5891521	-	297	WP_015150732.1	DUF1778 domain-containing protein	Antitoxin
OSCIL6304_RS22695	5891770..5892852	+	1083	WP_015149763.1	photosystem II q(b) protein	-
OSCIL6304_RS22700	5892953..5893594	-	642	WP_015150733.1	ribosome-associated translation inhibitor RaiA	-
OSCIL6304_RS22705	5894365..5895081	+	717	WP_015150735.1	lipoyl(octanoyl) transferase LipB	-
OSCIL6304_RS22710	5895437..5895796	+	360	WP_015150736.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (77-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (11-98)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 18552.27 Da
• Isoelectric Point: 8.7090

>T28521 WP_015150731.1 NC_019693:c5891234-5890716 [Oscillatoria acuminata PCC 6304]
VGLDSDALRPPEKLNSSHQIERFDSGNSSLNDWLKRRGLKNEAEGASRTYVLCAGDVVIAYYCLANGAVSQITATGRSRR
NMPDPIPVMVIGRLAVDRHWQGKGIGSALLRDAILRTLQAAEIAGIRAILVHAISEEAKQFYEKCGFTASAIDPMTLMVT
VKDAKNALGLSG

Nucleotide

• Length: 519 bp

>T28521 NC_019693:c5891234-5890716 [Oscillatoria acuminata PCC 6304]
GTGGGATTAGATAGCGACGCGCTTCGCCCTCCGGAAAAGCTAAATTCGTCCCATCAAATAGAGCGCTTTGACTCAGGAAA
TAGCTCGCTTAATGACTGGCTCAAACGCCGTGGGTTAAAAAATGAAGCTGAGGGGGCTTCGCGAACCTATGTTCTCTGCG
CGGGGGACGTAGTGATTGCATATTACTGTCTTGCAAATGGGGCTGTATCCCAAATTACTGCAACGGGTCGATCGCGGAGG
AATATGCCCGATCCGATTCCGGTGATGGTGATTGGAAGACTGGCTGTCGATCGCCACTGGCAAGGCAAAGGGATCGGGAG
TGCACTGTTACGAGATGCTATTCTTCGTACCCTGCAAGCTGCTGAAATTGCAGGAATTCGGGCAATTCTTGTTCATGCGA
TTTCGGAAGAAGCCAAGCAGTTTTATGAAAAGTGTGGGTTTACTGCTTCTGCGATCGATCCGATGACACTAATGGTGACG
GTTAAAGATGCGAAAAATGCACTAGGTTTGTCAGGCTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11317.59 Da
• Isoelectric Point: 6.2214

>AT28521 WP_015150732.1 NC_019693:c5891521-5891225 [Oscillatoria acuminata PCC 6304]
MSRSEFEYSSRQTRDVTINIRATQSQRDLIDQAAHIQGKSRSEFMLESAYQKAQDALLERTFFGLDDQKYQEFVALLDAG
PQPNAKLHHLLTTKSPWD

Nucleotide

• Length: 297 bp

>AT28521 NC_019693:c5891521-5891225 [Oscillatoria acuminata PCC 6304]
ATGTCTCGCTCAGAATTTGAATACTCATCTCGCCAAACTCGGGATGTAACGATCAACATCCGAGCCACGCAGAGTCAGAG
GGATCTCATTGATCAAGCGGCTCACATCCAGGGAAAAAGCCGGTCCGAGTTTATGCTTGAATCAGCCTATCAAAAAGCAC
AAGATGCTTTGCTAGAGCGGACTTTTTTTGGGTTAGATGACCAAAAATATCAGGAATTTGTCGCGCTGCTCGATGCAGGC
CCTCAGCCAAACGCCAAGTTACACCATCTTTTAACCACTAAATCTCCGTGGGATTAG

Structures

Toxin

• AlphaFold DB: K9TPL3

Antitoxin

• AlphaFold DB: K9TML6