Detailed information of TA system TA028519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1005927..1006431
• Replicon: chromosome
• Accession: NC_019693
• Organism: Oscillatoria acuminata PCC 6304

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: K9TDD5
• Locus tag: OSCIL6304_RS04025
• Protein ID: WP_015147200.1
• Coordinates: 1006153..1006431 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: OSCIL6304_RS04020
• Protein ID: WP_044194458.1
• Coordinates: 1005927..1006133 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OSCIL6304_RS03995	1001188..1001670	+	483	WP_015147194.1	photosystem I reaction centre subunit III	-
OSCIL6304_RS32155	1001761..1001886	+	126	WP_015147195.1	Photosystem I reaction centre subunit IX / PsaJ	-
OSCIL6304_RS04000	1002170..1002730	-	561	WP_015147196.1	guanylate kinase	-
OSCIL6304_RS04005	1002854..1003105	-	252	WP_015147197.1	DUF370 domain-containing protein	-
OSCIL6304_RS04010	1003614..1005365	+	1752	WP_015147198.1	fibronectin-binding domain-containing protein	-
OSCIL6304_RS04020	1005927..1006133	-	207	WP_044194458.1	BrnA antitoxin family protein	Antitoxin
OSCIL6304_RS04025	1006153..1006431	-	279	WP_015147200.1	BrnT family toxin	Toxin
OSCIL6304_RS04030	1006981..1008702	+	1722	WP_015147201.1	ABC transporter ATP-binding protein/permease	-
OSCIL6304_RS04035	1009013..1009285	+	273	WP_198017880.1	BrnT family toxin	-
OSCIL6304_RS04040	1009272..1009559	+	288	WP_015147203.1	hypothetical protein	-
OSCIL6304_RS04045	1009658..1010074	+	417	WP_015147204.1	hypothetical protein	-
OSCIL6304_RS04050	1010084..1010458	+	375	WP_015147205.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (10-64)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10964.33 Da
• Isoelectric Point: 5.7362

>T28519 WP_015147200.1 NC_019693:c1006431-1006153 [Oscillatoria acuminata PCC 6304]
MQFEGDEAKRAENIVKHQIDFADIPQMFDGSMLSELDQRTDYGEERWVGIGFLRNTVAVVVWTERKHNIIRIISARRANK
YERERFQQYLSD

Nucleotide

• Length: 279 bp

>T28519 NC_019693:c1006431-1006153 [Oscillatoria acuminata PCC 6304]
ATGCAGTTTGAAGGGGACGAAGCGAAGAGAGCGGAAAACATTGTCAAACATCAGATTGACTTCGCAGATATTCCTCAGAT
GTTTGATGGCTCCATGTTAAGTGAGCTTGATCAGCGTACTGATTATGGCGAGGAGCGGTGGGTGGGTATCGGCTTCTTGC
GAAATACTGTAGCGGTTGTTGTCTGGACAGAACGAAAGCATAATATAATTAGAATTATATCAGCACGAAGAGCTAATAAA
TATGAGCGAGAAAGATTTCAACAATACCTCTCGGACTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7927.77 Da
• Isoelectric Point: 4.1796

>AT28519 WP_044194458.1 NC_019693:c1006133-1005927 [Oscillatoria acuminata PCC 6304]
MSDEEIDYSDIPPLTDEFFENATLRIPATQARNLIQLDADVMAWFRGQGSEYRNTINSVLRRYIESSR

Nucleotide

• Length: 207 bp

>AT28519 NC_019693:c1006133-1005927 [Oscillatoria acuminata PCC 6304]
ATGAGTGATGAGGAAATTGATTACTCGGATATTCCCCCTTTAACGGATGAGTTTTTTGAGAACGCTACTCTCAGAATACC
AGCGACCCAAGCTCGTAATTTGATTCAGTTAGATGCGGATGTGATGGCATGGTTTCGAGGGCAAGGGTCAGAGTATAGAA
ACACGATTAATTCAGTGTTGCGGCGTTATATAGAGAGTAGCAGATAG

Structures

Toxin

• AlphaFold DB: K9TDD5