Detailed information of TA system TA028518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 293794..294717
• Replicon: plasmid pPSE7367.01
• Accession: NC_019690
• Organism: Pseudanabaena sp. PCC 7367

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: PSE7367_RS19680
• Protein ID: WP_015146308.1
• Coordinates: 293794..294369 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: PSE7367_RS19685
• Protein ID: WP_051038210.1
• Coordinates: 294379..294717 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSE7367_RS19665	289380..291107	-	1728	WP_015146305.1	diflavin flavoprotein	-
PSE7367_RS19670	291924..292811	+	888	WP_051038202.1	HEAT repeat domain-containing protein	-
PSE7367_RS19675	293001..293771	+	771	WP_015146307.1	HEAT repeat domain-containing protein	-
PSE7367_RS19680	293794..294369	-	576	WP_015146308.1	PIN domain-containing protein	Toxin
PSE7367_RS19685	294379..294717	-	339	WP_051038210.1	excisionase family DNA-binding protein	Antitoxin
PSE7367_RS19690	295879..296070	+	192	WP_015146311.1	hypothetical protein	-
PSE7367_RS19695	296085..296600	-	516	WP_015146312.1	hypothetical protein	-
PSE7367_RS19700	296638..298467	-	1830	WP_015146313.1	L-aspartate oxidase	-
PSE7367_RS19705	298594..299166	-	573	WP_041700306.1	Uma2 family endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..328634	328634

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• Helix-turn-helix domain, group 17 (41-89)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 22145.84 Da
• Isoelectric Point: 6.8394

>T28518 WP_015146308.1 NC_019690:c294369-293794 [Pseudanabaena sp. PCC 7367]
MIRDMAILDANILFSGPMRYMFVELALTAGFFQVKWTEDIHREWIKNLLKKRPDLDRNYLEKTRQLMNNKVRDCLVFDYE
PIIPTLNLPDPNDRHVLAAAIAGDCNVIVTKNLKDFPAQLLAQYGIRAEHPDSFLCEHLALETALFCQCTKKIRTEYYQN
PPYSVSEYLAILKKQGLIQLVSALERLTELI

Nucleotide

• Length: 576 bp

>T28518 NC_019690:c294369-293794 [Pseudanabaena sp. PCC 7367]
ATGATTCGAGATATGGCGATCCTTGATGCAAATATCCTTTTCTCTGGGCCAATGCGATATATGTTTGTGGAATTGGCACT
TACCGCTGGCTTTTTCCAAGTGAAATGGACAGAAGATATTCACCGCGAATGGATAAAAAATCTTCTCAAAAAGAGACCTG
ATTTAGATCGTAATTATCTTGAGAAAACCCGCCAACTCATGAACAATAAAGTTCGTGATTGCTTAGTATTTGATTATGAA
CCAATAATCCCGACCCTGAATCTGCCCGATCCCAATGATCGACATGTATTGGCAGCGGCGATCGCAGGTGATTGCAATGT
AATTGTCACCAAAAACCTAAAAGATTTTCCAGCTCAGCTTCTTGCTCAATATGGAATTAGGGCAGAACATCCAGATAGTT
TTTTATGTGAACATCTAGCATTAGAGACAGCGCTCTTTTGTCAATGCACTAAAAAAATCCGCACCGAGTATTATCAAAAT
CCACCCTATTCTGTGTCAGAGTATTTAGCAATTCTTAAAAAACAGGGGCTAATTCAACTGGTTTCTGCTCTAGAGCGATT
GACTGAACTGATTTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12468.41 Da
• Isoelectric Point: 6.2294

>AT28518 WP_051038210.1 NC_019690:c294717-294379 [Pseudanabaena sp. PCC 7367]
MLSLGQESIELPAATIAMLEQLLEAIAQGKSVYIIPEHAELSTFEAADLLNVSRPYLIKLLNAGEIPYHKVGSHRRIKMK
NLSAYKDKDDAERAQAIKQLIAEDQKYGLVGY

Nucleotide

• Length: 339 bp

>AT28518 NC_019690:c294717-294379 [Pseudanabaena sp. PCC 7367]
ATGTTGAGCCTAGGTCAAGAATCGATCGAATTACCAGCCGCCACAATCGCCATGCTGGAGCAGTTGTTAGAAGCGATCGC
CCAGGGCAAAAGTGTCTATATCATTCCTGAACATGCGGAACTGAGTACCTTTGAAGCCGCCGATCTGCTCAACGTCTCGC
GTCCTTATTTGATTAAGCTGCTCAATGCGGGTGAAATCCCCTATCACAAGGTGGGCAGTCATCGCCGGATCAAGATGAAA
AATTTGAGCGCGTATAAAGACAAAGACGACGCGGAGAGAGCACAGGCTATAAAACAACTTATTGCGGAAGATCAAAAGTA
TGGCTTGGTGGGATATTAA