Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-ChpS
Location 535204..535790 Replicon plasmid pHAMBI1141a
Accession NZ_HG938356
Organism Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI 1141

Toxin (Protein)


Gene name pemK Uniprot ID A0A068TFX5
Locus tag RG1141_RS24995 Protein ID WP_040124486.1
Coordinates 535461..535790 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A068TH23
Locus tag RG1141_RS24990 Protein ID WP_040125728.1
Coordinates 535204..535461 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG1141_RS24985 533355..535148 + 1794 WP_040124485.1 acyl-CoA dehydrogenase C-terminal domain-containing protein -
RG1141_RS24990 535204..535461 + 258 WP_040125728.1 antitoxin Antitoxin
RG1141_RS24995 535461..535790 + 330 WP_040124486.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
RG1141_RS25000 535842..537050 + 1209 WP_040125729.1 acetyl-CoA C-acetyltransferase -
RG1141_RS25005 537226..539442 + 2217 WP_040124487.1 enoyl-CoA hydratase/isomerase family protein -
RG1141_RS25010 539726..540004 - 279 WP_040124488.1 BrnA antitoxin family protein -
RG1141_RS25015 539988..540290 - 303 WP_040124490.1 BrnT family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB / htpB 1..1638739 1638739


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11972.73 Da        Isoelectric Point: 9.1969

>T285174 WP_040124486.1 NZ_HG938356:535461-535790 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
MERGDIWHVDLEPIKGREQAGSRYALIVTQKSFNNVGTPVIVPITTGGQFARFQGFAVSLSGAGTRSSGVVLCNQLRAVD
LKARGARFVERVPDFIMDEVLARISTFFE

Download         Length: 330 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9196.49 Da        Isoelectric Point: 4.0610

>AT285174 WP_040125728.1 NZ_HG938356:535204-535461 [Neorhizobium galegae bv. officinalis bv. officinalis str. HAMBI]
MASSTLRNVGGSVMMAIPKPVLEELGLSANTKLEVSAEGGRIVASPQTRPKYTLDELLAQCDLDAPFTEEDLEWLNDPPV
GREII

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A068TFX5

Antitoxin

Source ID Structure
AlphaFold DB A0A068TH23

References