Detailed information of TA system TA028517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 41256..41889
• Replicon: plasmid pPSE7367.01
• Accession: NC_019690
• Organism: Pseudanabaena sp. PCC 7367

Toxin (Protein)

• Gene name: tad
• Uniprot ID: K9SNZ3
• Locus tag: PSE7367_RS18535
• Protein ID: WP_015146092.1
• Coordinates: 41256..41582 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: PSE7367_RS18540
• Protein ID: WP_015146093.1
• Coordinates: 41572..41889 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSE7367_RS18515	36958..37173	-	216	WP_015146088.1	hypothetical protein	-
PSE7367_RS18520	37592..38518	-	927	WP_015146089.1	plasmid encoded RepA protein	-
PSE7367_RS21345	38865..40346	+	1482	Protein_32	IS21 family transposase	-
PSE7367_RS18530	40333..41103	+	771	WP_083883792.1	IS21-like element helper ATPase IstB	-
PSE7367_RS18535	41256..41582	+	327	WP_015146092.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSE7367_RS18540	41572..41889	+	318	WP_015146093.1	helix-turn-helix domain-containing protein	Antitoxin
PSE7367_RS18545	42251..43057	+	807	WP_015146094.1	ParA family protein	-
PSE7367_RS18550	43054..43455	+	402	WP_015146095.1	hypothetical protein	-
PSE7367_RS18555	43488..43868	+	381	WP_015146096.1	four helix bundle protein	-
PSE7367_RS18560	44251..45480	+	1230	WP_071881412.1	DNA (cytosine-5-)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..328634	328634
	-	flank	IS/Tn	-	-	40489..41103	614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (7-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-83)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12209.10 Da
• Isoelectric Point: 10.2016

>T28517 WP_015146092.1 NC_019690:41256-41582 [Pseudanabaena sp. PCC 7367]
MGDSRKRLKEFPKDVQVTMGGALMIAQYGGMADNVKPLKGFGSGVFEIVDRYDKNAYRLVYAVQIGEQIYVLHAFQKKSK
QGISTPKADLDLIKRRYKDAQEAAKHDR

Nucleotide

• Length: 327 bp

>T28517 NC_019690:41256-41582 [Pseudanabaena sp. PCC 7367]
ATGGGGGACTCCAGAAAAAGGCTCAAAGAGTTTCCTAAGGATGTTCAGGTCACCATGGGCGGTGCATTGATGATTGCCCA
GTATGGGGGCATGGCTGATAATGTTAAGCCACTCAAAGGTTTTGGCAGTGGTGTATTTGAGATTGTCGATCGCTATGACA
AAAATGCTTATCGGTTGGTCTATGCCGTCCAAATTGGTGAGCAGATATATGTTTTACATGCTTTTCAGAAAAAGTCAAAG
CAAGGCATATCAACGCCAAAAGCAGATTTGGATCTGATTAAACGCCGTTACAAGGATGCACAGGAGGCCGCAAAACATGA
CCGTTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11849.56 Da
• Isoelectric Point: 4.8430

>AT28517 WP_015146093.1 NC_019690:41572-41889 [Pseudanabaena sp. PCC 7367]
MTVDYETSSGNVFADLGLDNADELFIRAQLGIQVFKIIRERNLTQKDAAELLGIKQPEVSAIMCGKFDRFSQERLIGFLN
RLEQKVVIQVSPHLEGEPYQHVAIS

Nucleotide

• Length: 318 bp

>AT28517 NC_019690:41572-41889 [Pseudanabaena sp. PCC 7367]
ATGACCGTTGATTATGAAACCAGCAGTGGCAATGTTTTTGCCGATCTTGGCCTCGATAATGCCGATGAGTTGTTCATCCG
TGCTCAGCTTGGAATTCAGGTGTTTAAAATTATCCGCGAGCGTAATCTCACCCAGAAAGATGCTGCAGAGCTCCTGGGCA
TTAAACAGCCTGAGGTTTCAGCAATTATGTGTGGCAAATTCGATCGCTTTAGCCAGGAAAGACTTATTGGCTTTCTAAAC
CGGTTAGAGCAGAAAGTAGTAATCCAAGTCAGCCCGCATCTAGAGGGGGAACCTTATCAACATGTTGCCATCTCGTAA

Structures

Toxin

• AlphaFold DB: K9SNZ3