Detailed information of TA system TA028516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-ParD
• Location: 2357197..2357746
• Replicon: chromosome
• Accession: NC_019689
• Organism: Pleurocapsa sp. PCC 7327

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K9T515
• Locus tag: PLE7327_RS10510
• Protein ID: WP_015143811.1
• Coordinates: 2357197..2357535 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9T666
• Locus tag: PLE7327_RS10515
• Protein ID: WP_015143812.1
• Coordinates: 2357522..2357746 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLE7327_RS24820	2352462..2352623	-	162	WP_186005377.1	hypothetical protein	-
PLE7327_RS10485	2352808..2353605	+	798	WP_041393125.1	SAM-dependent chlorinase/fluorinase	-
PLE7327_RS10490	2353602..2354243	-	642	WP_015143807.1	response regulator transcription factor	-
PLE7327_RS10495	2354240..2355145	-	906	WP_015143808.1	trypsin-like peptidase domain-containing protein	-
PLE7327_RS10500	2355294..2356214	-	921	WP_015143809.1	S1C family serine protease	-
PLE7327_RS24340	2356433..2356561	+	129	Protein_2102	DUF2281 domain-containing protein	-
PLE7327_RS10505	2356722..2357118	+	397	Protein_2103	type II toxin-antitoxin system VapC family toxin	-
PLE7327_RS10510	2357197..2357535	-	339	WP_015143811.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PLE7327_RS10515	2357522..2357746	-	225	WP_015143812.1	hypothetical protein	Antitoxin
PLE7327_RS24350	2358164..2358295	+	132	Protein_2106	ABC transporter ATP-binding protein	-
PLE7327_RS10520	2358405..2359229	+	825	WP_015143814.1	glycosyltransferase	-
PLE7327_RS10525	2359493..2360671	+	1179	WP_015143815.1	glycosyltransferase family 4 protein	-
PLE7327_RS10530	2360772..2360981	-	210	WP_015143816.1	type II toxin-antitoxin system HicA family toxin	-
PLE7327_RS10535	2360978..2361190	-	213	WP_015143817.1	type II toxin-antitoxin system HicB family antitoxin	-
PLE7327_RS10540	2361311..2362060	+	750	WP_144266194.1	WecB/TagA/CpsF family glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12425.41 Da
• Isoelectric Point: 9.5622

>T28516 WP_015143811.1 NC_019689:c2357535-2357197 [Pleurocapsa sp. PCC 7327]
MKRGEIYYANLSPTVGSEIAKRRPVLIVSDDINNHAASTVTVLPITSNLNRVYPFEVLLQPSDSGLPKPSKVQAQQVRTI
SKQRITGEMMGNLREELMQLVDAALKLHLALE

Nucleotide

• Length: 339 bp

>T28516 NC_019689:c2357535-2357197 [Pleurocapsa sp. PCC 7327]
ATGAAACGTGGTGAAATCTACTACGCTAATCTTAGCCCCACTGTTGGATCTGAGATAGCTAAACGCCGTCCAGTGTTGAT
AGTCAGCGATGATATTAATAACCATGCTGCTTCTACAGTGACAGTTTTACCGATTACATCAAATCTCAATCGCGTCTACC
CTTTTGAGGTTCTTCTTCAGCCATCTGATAGCGGTTTGCCCAAGCCTTCTAAAGTTCAGGCGCAACAGGTAAGAACGATT
TCTAAACAGCGAATTACTGGGGAGATGATGGGAAATTTAAGAGAGGAATTAATGCAGTTAGTCGATGCAGCTTTAAAGCT
TCATTTGGCACTAGAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8474.47 Da
• Isoelectric Point: 4.1322

>AT28516 WP_015143812.1 NC_019689:c2357746-2357522 [Pleurocapsa sp. PCC 7327]
MQSQKLSISLSPSLVRFIESYRVAKGCKSRSQVIEEALTLLQERELEEAYREASQEIDPDWELTVADGLADETW

Nucleotide

• Length: 225 bp

>AT28516 NC_019689:c2357746-2357522 [Pleurocapsa sp. PCC 7327]
ATGCAGTCGCAAAAATTATCAATTTCCTTATCACCTTCCCTAGTAAGGTTTATTGAGAGTTATAGGGTTGCCAAAGGATG
TAAATCTCGCTCTCAAGTGATTGAGGAAGCTTTAACCTTATTACAAGAACGAGAATTAGAAGAAGCTTACAGAGAAGCCT
CTCAAGAAATCGATCCAGATTGGGAGTTGACAGTTGCGGATGGGTTAGCTGATGAAACGTGGTGA

Structures

Toxin

• AlphaFold DB: K9T515

Antitoxin

• AlphaFold DB: K9T666