Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 1378386..1379059 Replicon plasmid pHAMBI540a
Accession NZ_HG938354
Organism Neorhizobium galegae bv. orientalis str. HAMBI 540

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag RG540_RS29130 Protein ID WP_041365717.1
Coordinates 1378655..1379059 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A068T0M6
Locus tag RG540_RS29125 Protein ID WP_041365715.1
Coordinates 1378386..1378658 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG540_RS29105 1373445..1375043 - 1599 WP_046601468.1 ABC transporter substrate-binding protein -
RG540_RS29110 1375165..1375932 - 768 WP_080725132.1 GntR family transcriptional regulator -
RG540_RS29115 1376023..1377270 + 1248 WP_041365713.1 glucarate dehydratase family protein -
RG540_RS29120 1377333..1377826 + 494 Protein_1342 ribonuclease activity regulator RraA -
RG540_RS32165 1377949..1378068 - 120 Protein_1343 helix-turn-helix transcriptional regulator -
RG540_RS29125 1378386..1378658 + 273 WP_041365715.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
RG540_RS29130 1378655..1379059 + 405 WP_041365717.1 type II toxin-antitoxin system VapC family toxin Toxin
RG540_RS29135 1379302..1379667 + 366 Protein_1346 transposase -
RG540_RS32695 1379670..1379792 - 123 Protein_1347 IS3 family transposase -
RG540_RS29140 1379905..1381086 - 1182 WP_041365719.1 DNA-binding transcriptional regulator -
RG540_RS29145 1381300..1382553 + 1254 WP_046601469.1 extracellular solute-binding protein -
RG540_RS29150 1382619..1383551 + 933 WP_041365724.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ddhA / htpB 1..1807065 1807065


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-124)

Antitoxin

SpoVT-AbrB domain

(16-54)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14745.10 Da        Isoelectric Point: 4.7581

>T285152 WP_041365717.1 NZ_HG938354:1378655-1379059 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
MTGYLLDTNIISDIIRNPFGSAARRVEEIDPKEICTSIVVAAELRYGCAKKGSTKLLAKVESILETIPIMPLDMPADINY
GGIRAKLEAAGETIGLNDMLIAAHACALNLTLVTDNTREFQRIRGLDLENWLER

Download         Length: 405 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10316.94 Da        Isoelectric Point: 4.8952

>AT285152 WP_041365715.1 NZ_HG938354:1378386-1378658 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
VPIPLPSSRPKEVKLFRNNRSQAVRIPAEFELPGDRVLIHREGNKLIIEPIARPANIIELLAEWKKEDLLGPEDQFPDIE
DMPAKPENIF

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A068T0M6

References