Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 253314..253999 Replicon plasmid pHAMBI540a
Accession NZ_HG938354
Organism Neorhizobium galegae bv. orientalis str. HAMBI 540

Toxin (Protein)


Gene name vapC Uniprot ID A0A068T0F6
Locus tag RG540_RS23785 Protein ID WP_041364108.1
Coordinates 253314..253736 (-) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A068SYQ3
Locus tag RG540_RS23790 Protein ID WP_041364111.1
Coordinates 253733..253999 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG540_RS23765 248699..249619 - 921 WP_041364102.1 ABC transporter permease -
RG540_RS23770 249661..251181 - 1521 WP_041364103.1 ABC transporter substrate-binding protein -
RG540_RS23775 251393..252178 + 786 WP_041364104.1 IclR family transcriptional regulator -
RG540_RS23780 252251..252973 + 723 WP_041364107.1 ribonuclease activity regulator RraA -
RG540_RS23785 253314..253736 - 423 WP_041364108.1 type II toxin-antitoxin system VapC family toxin Toxin
RG540_RS23790 253733..253999 - 267 WP_041364111.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RG540_RS23795 254088..254528 - 441 WP_041364114.1 fucose-binding protein -
RG540_RS23800 254893..255954 + 1062 WP_041364117.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
RG540_RS23805 256031..257692 + 1662 WP_041364118.1 sugar ABC transporter substrate-binding protein -
RG540_RS23810 257803..258912 + 1110 WP_041364121.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ddhA / htpB 1..1807065 1807065


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-63)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15427.67 Da        Isoelectric Point: 4.9436

>T285150 WP_041364108.1 NZ_HG938354:c253736-253314 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
VRLLIDTNVLPEVTKPRPDARVLEWLGGLDEDRAYISVISIAEIRRGVALMDSGQKRDTLAEWLSHDLPQRFEGRVIPVE
EPVALSWGDLMALAKQSGRGLASMDGLIAATAIAHHLTLATRNTKDFEGFGIDMINPWNG

Download         Length: 423 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9808.13 Da        Isoelectric Point: 8.8061

>AT285150 WP_041364111.1 NZ_HG938354:c253999-253733 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
MRPSAHDDRIWTVAGAKAKLSEVIERAQSVPQTITRNGKPSVVVVSAEEWQRKTARKGSLAEFLMDSPLRGAGLDLDRER
DEPRDLPL

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A068T0F6

Antitoxin

Source ID Structure
AlphaFold DB A0A068SYQ3

References