Detailed information of TA system TA028515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 6269285..6269868
• Replicon: chromosome
• Accession: NC_019684
• Organism: Nostoc sp. PCC 7524 (Nostoc sp. ATCC 29411)

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K9QZW3
• Locus tag: NOS7524_RS25660
• Protein ID: WP_015141397.1
• Coordinates: 6269521..6269868 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9R1L2
• Locus tag: NOS7524_RS25655
• Protein ID: WP_015141396.1
• Coordinates: 6269285..6269527 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NOS7524_RS25635	6264852..6265997	+	1146	WP_015141392.1	STM4014 family protein	-
NOS7524_RS25640	6265985..6266785	+	801	WP_015141393.1	STM4013/SEN3800 family hydrolase	-
NOS7524_RS25645	6266864..6268198	+	1335	WP_015141394.1	STM4012 family radical SAM protein	-
NOS7524_RS25650	6268254..6269132	+	879	WP_015141395.1	STM4011 family radical SAM protein	-
NOS7524_RS25655	6269285..6269527	+	243	WP_015141396.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
NOS7524_RS25660	6269521..6269868	+	348	WP_015141397.1	endoribonuclease MazF	Toxin
NOS7524_RS25665	6270020..6271135	+	1116	WP_015141398.1	PD-(D/E)XK nuclease family protein	-
NOS7524_RS25670	6271297..6271644	-	348	WP_015141399.1	type II toxin-antitoxin system RelE/ParE family toxin	-
NOS7524_RS25675	6271641..6271895	-	255	WP_015141400.1	hypothetical protein	-
NOS7524_RS25680	6271944..6273341	-	1398	WP_015141401.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (12-112)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13056.22 Da
• Isoelectric Point: 7.9790

>T28515 WP_015141397.1 NC_019684:6269521-6269868 [Nostoc sp. PCC 7524]
MVTTANFYVPSRGDIVYLDFDPTKGHEQQGYRPAFVISPRRYNEKSSLALFMPITKQQKGYPFEVLLPSDLKIQGVILAD
QIKCLDWKVRGIKFIESAPESVIAEVQAKIEPLLL

Nucleotide

• Length: 348 bp

>T28515 NC_019684:6269521-6269868 [Nostoc sp. PCC 7524]
TTGGTAACAACAGCAAATTTCTATGTCCCCAGTCGGGGAGATATTGTCTACTTAGATTTTGATCCCACAAAAGGACATGA
ACAACAAGGATATAGGCCTGCTTTTGTAATCTCACCTCGCAGGTACAACGAGAAAAGTTCTTTGGCTTTATTTATGCCGA
TTACAAAGCAGCAAAAAGGATATCCTTTTGAGGTTCTTTTACCGTCTGATTTGAAAATTCAAGGTGTAATTCTTGCGGAT
CAGATCAAGTGTTTAGACTGGAAAGTACGCGGGATTAAATTTATTGAGTCTGCACCAGAAAGTGTCATTGCAGAAGTGCA
AGCAAAAATTGAGCCATTACTTTTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8704.99 Da
• Isoelectric Point: 4.7469

>AT28515 WP_015141396.1 NC_019684:6269285-6269527 [Nostoc sp. PCC 7524]
MAATVAKWGNSLAIRIPQNLAKEIHLTEGVEVELAVIDGSLVIKPKTRKRYSLDELIDAITPENVHGEIDSGIAVGNEVW

Nucleotide

• Length: 243 bp

>AT28515 NC_019684:6269285-6269527 [Nostoc sp. PCC 7524]
ATGGCTGCGACTGTTGCCAAGTGGGGAAATAGTCTTGCGATTAGGATTCCCCAGAATTTAGCGAAAGAGATTCATTTAAC
CGAAGGGGTAGAAGTAGAGCTTGCTGTCATAGATGGTAGCCTAGTAATTAAACCCAAAACCCGCAAGCGATACTCACTTG
ATGAGTTAATTGATGCTATTACACCAGAGAATGTTCATGGTGAGATTGATAGTGGAATAGCTGTGGGGAATGAGGTTTGG
TAA

Structures

Toxin

• AlphaFold DB: K9QZW3

Antitoxin

• AlphaFold DB: K9R1L2