Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09812-MazE
Location 172573..173207 Replicon plasmid pHAMBI540a
Accession NZ_HG938354
Organism Neorhizobium galegae bv. orientalis str. HAMBI 540

Toxin (Protein)


Gene name mazF Uniprot ID A0A068SYI0
Locus tag RG540_RS23380 Protein ID WP_041363971.1
Coordinates 172573..172944 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A068SX90
Locus tag RG540_RS23385 Protein ID WP_041363973.1
Coordinates 172944..173207 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG540_RS23350 168023..169600 + 1578 WP_041363961.1 ATP-binding cassette domain-containing protein -
RG540_RS23355 169607..170164 - 558 WP_041366245.1 isochorismatase family protein -
RG540_RS23360 170388..170621 - 234 WP_041363963.1 hypothetical protein -
RG540_RS23365 170781..171563 - 783 WP_041363966.1 SDR family oxidoreductase -
RG540_RS23370 171654..172073 - 420 WP_041363967.1 HD domain-containing protein -
RG540_RS23375 172133..172507 - 375 WP_041363969.1 GFA family protein -
RG540_RS23380 172573..172944 - 372 WP_041363971.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
RG540_RS23385 172944..173207 - 264 WP_041363973.1 PbsX family transcriptional regulator Antitoxin
RG540_RS23390 173374..173877 + 504 WP_041366246.1 hypothetical protein -
RG540_RS23395 173901..175277 - 1377 WP_041363976.1 FAD-binding protein -
RG540_RS23400 175274..176929 - 1656 WP_041363978.1 thiamine pyrophosphate-requiring protein -
RG540_RS23405 176959..178191 - 1233 WP_041363981.1 alpha-hydroxy-acid oxidizing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ddhA / htpB 1..1807065 1807065


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13449.41 Da        Isoelectric Point: 6.7170

>T285149 WP_041363971.1 NZ_HG938354:c172944-172573 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
MIHSNVPRRGDVYWIDPNPVAGREMKNRHRFVVITPIEINRLGVSMTVPITTGGAFIRDVGLAVAISGHDTTGVAVCNQV
RSFDIPARIQQKTAQYIETLDEATMNEIVSRVVSAIDPAPEPA

Download         Length: 372 bp

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9233.69 Da        Isoelectric Point: 5.6492

>AT285149 WP_041363973.1 NZ_HG938354:c173207-172944 [Neorhizobium galegae bv. orientalis str. HAMBI 540]
MPVTTKIRRQGGAAVITIPPALLKLMHTEVGDQLTLDVANGELIARPVESGRKRYTLSELLKGSDAMAMLNAAVADAQEG
DPIGHEI

Download         Length: 264 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A068SYI0

Antitoxin

Source ID Structure
AlphaFold DB A0A068SX90

References