Detailed information of TA system TA028514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 4688541..4689151
• Replicon: chromosome
• Accession: NC_019684
• Organism: Nostoc sp. PCC 7524 (Nostoc sp. ATCC 29411)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: K9QVK8
• Locus tag: NOS7524_RS18835
• Protein ID: WP_015140072.1
• Coordinates: 4688765..4689151 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: K9QX39
• Locus tag: NOS7524_RS18830
• Protein ID: WP_015140071.1
• Coordinates: 4688541..4688768 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NOS7524_RS18795	4683732..4685522	+	1791	WP_015140064.1	translational GTPase TypA	-
NOS7524_RS18800	4685694..4686518	+	825	WP_015140065.1	hypothetical protein	-
NOS7524_RS18805	4686734..4686973	+	240	WP_144050955.1	DUF4258 domain-containing protein	-
NOS7524_RS18810	4686978..4687220	+	243	WP_015140067.1	YgiT-type zinc finger protein	-
NOS7524_RS29590	4687282..4687455	+	174	WP_015140068.1	hypothetical protein	-
NOS7524_RS18820	4687659..4687955	+	297	WP_015140069.1	BrnT family toxin	-
NOS7524_RS18825	4687945..4688229	+	285	WP_015140070.1	hypothetical protein	-
NOS7524_RS18830	4688541..4688768	+	228	WP_015140071.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
NOS7524_RS18835	4688765..4689151	+	387	WP_015140072.1	type II toxin-antitoxin system VapC family toxin	Toxin
NOS7524_RS18840	4689284..4690444	+	1161	WP_041555386.1	DUF58 domain-containing protein	-
NOS7524_RS18845	4690651..4691016	+	366	WP_015140074.1	ferredoxin:thioredoxin reductase	-
NOS7524_RS18850	4691013..4691405	+	393	WP_015140075.1	DUF309 domain-containing protein	-
NOS7524_RS18855	4691590..4692090	-	501	WP_015140076.1	hypothetical protein	-
NOS7524_RS18860	4692281..4692778	+	498	WP_041555387.1	OstA family protein	-
NOS7524_RS18865	4692807..4693538	+	732	WP_015140078.1	LPS export ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

• Domain of unknown function DUF2281 (48-73)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14939.39 Da
• Isoelectric Point: 5.1514

>T28514 WP_015140072.1 NC_019684:4688765-4689151 [Nostoc sp. PCC 7524]
MRLMLDTHTFLWFIEGSLNLSDTAKNLIEDQRNQRFLSIASLWEMSIKVSIGKLQLGMNFAELVKQQVYGNAIELLEIRP
EHLDKLAQLPFYHKDPFDRLIISQSLVESIPIVTKDSMFESYPVQILW

Nucleotide

• Length: 387 bp

>T28514 NC_019684:4688765-4689151 [Nostoc sp. PCC 7524]
ATGAGGCTCATGCTCGATACCCATACGTTTTTATGGTTTATTGAGGGCAGTTTGAATCTCAGCGATACGGCAAAAAACCT
AATTGAAGATCAGCGAAATCAAAGGTTTTTGAGCATTGCCAGTCTTTGGGAAATGTCTATCAAAGTCAGTATTGGCAAGC
TACAACTAGGTATGAATTTTGCTGAGTTGGTGAAGCAACAGGTTTATGGTAACGCTATTGAATTACTTGAAATACGACCA
GAACATTTAGATAAGTTAGCACAGCTGCCTTTTTACCATAAAGATCCATTTGATCGGCTGATAATTTCTCAAAGTCTGGT
GGAGAGCATACCTATAGTTACTAAAGATAGTATGTTTGAAAGCTATCCTGTGCAAATATTGTGGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8317.37 Da
• Isoelectric Point: 4.2815

>AT28514 WP_015140071.1 NC_019684:4688541-4688768 [Nostoc sp. PCC 7524]
MHQINLKEAEIRLAELIEEAASGEEVIITRSDGTSFKIVPLGRVEARPKFGSARGLVKISDDFDEPLADFEEYAP

Nucleotide

• Length: 228 bp

>AT28514 NC_019684:4688541-4688768 [Nostoc sp. PCC 7524]
ATGCACCAAATTAATTTGAAAGAAGCTGAGATTCGCCTAGCAGAACTAATTGAAGAGGCGGCTAGTGGAGAAGAAGTCAT
CATCACGCGCAGTGATGGCACATCTTTCAAAATTGTGCCGCTTGGTCGAGTGGAAGCACGCCCCAAGTTTGGTAGTGCTA
GGGGTTTAGTGAAAATCTCAGATGATTTTGATGAGCCGCTAGCAGATTTTGAGGAGTACGCGCCATGA

Structures

Toxin

• AlphaFold DB: K9QVK8

Antitoxin

• AlphaFold DB: K9QX39