Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 3379663..3381254 Replicon chromosome
Accession NZ_HG916826
Organism Pseudomonas pseudoalcaligenes CECT 5344 strain CECT5344

Toxin (Protein)


Gene name hipA Uniprot ID W6QYA1
Locus tag BN5_RS15805 Protein ID WP_003459717.1
Coordinates 3379929..3381254 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID W6RJ44
Locus tag BN5_RS15800 Protein ID WP_003459718.1
Coordinates 3379663..3379929 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BN5_RS15780 3375207..3375629 - 423 Protein_3144 NrdJb -
BN5_RS15785 3375819..3376622 - 804 WP_003459721.1 IS21-like element ISPst3 family helper ATPase IstB -
BN5_RS15790 3376615..3378114 - 1500 Protein_3146 IS21 family transposase -
BN5_RS23675 3378135..3378368 + 234 Protein_3147 IS4 family transposase -
BN5_RS15800 3379663..3379929 - 267 WP_003459718.1 helix-turn-helix domain-containing protein Antitoxin
BN5_RS15805 3379929..3381254 - 1326 WP_003459717.1 HipA domain-containing protein Toxin
BN5_RS15810 3381527..3381865 - 339 Protein_3150 transposase -
BN5_RS15815 3381885..3382094 - 210 WP_156157187.1 IS66 family insertion sequence element accessory protein TnpB -
BN5_RS15820 3382122..3383390 - 1269 WP_003459714.1 group II intron reverse transcriptase/maturase -
BN5_RS15825 3383797..3384102 - 306 Protein_3153 IS66 family insertion sequence element accessory protein TnpB -
BN5_RS15830 3384265..3385218 + 954 WP_003459712.1 IS1595-like element ISAchd1 family transposase -
BN5_RS15835 3385429..3385884 - 456 WP_003459711.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3346764..3385218 38454
- inside IScluster/Tn - - 3375819..3385218 9399


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-385)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 49143.75 Da        Isoelectric Point: 4.8860

>T285132 WP_003459717.1 NZ_HG916826:c3381254-3379929 [Pseudomonas pseudoalcaligenes CECT 5344]
MHNLTLQVYRDGQWRNAVELRFEQPEEGLEGACAYGYQHGYLVDSLESVGTRLNVAVSASYPLGWDSWRDKKAPAFLFDI
LPSGAARRFLLKRLGGERPEALNLDLYLLGRCTPAPIGNLRIKEAVEAIAGSPMLGFTRDEVISRDSRFLEYAYEQGAAI
GGATGAGGEAPKLLLTEDRQGALHPDAVLPDADAAQHWFVKFARNKASQTDQDILRSEYCFYRAVQQLGMDTVAAEGLAL
EEGNKPSLWMHRFDREISPAGVCRTAVESMYSLCGVTEAGSYMLHTQVLGKLVELWRAVGQEQQVGDLVFEYMRRDLLNQ
ILGNSDNHGRNTAILRSEQGVRLAPIYDLAPMVMDEEGVTRTTKWPKHIELAGEVNWRAACDEVAEWIDADELFQRLQLA
AQDLLALPDLLSAQGLPDSTMSHPRIALRNLQQRMLQWGLV

Download         Length: 1326 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9933.55 Da        Isoelectric Point: 10.9502

>AT285132 WP_003459718.1 NZ_HG916826:c3379929-3379663 [Pseudomonas pseudoalcaligenes CECT 5344]
MDILERNRILNELQQQLASGEITIGGAVRRLRKEVTGLQQARFAQMCKLSLRALRQLELDESNPTVQTLNSVFNPFGMQV
GIVPKTPR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W6QYA1

Antitoxin

Source ID Structure
AlphaFold DB W6RJ44

References