Detailed information of TA system TA028513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4573527..4574133
• Replicon: chromosome
• Accession: NC_019684
• Organism: Nostoc sp. PCC 7524 (Nostoc sp. ATCC 29411)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: K9QVA1
• Locus tag: NOS7524_RS18270
• Protein ID: WP_015139968.1
• Coordinates: 4573527..4573823 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: K9QWL0
• Locus tag: NOS7524_RS18275
• Protein ID: WP_015139969.1
• Coordinates: 4573813..4574133 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NOS7524_RS18250	4569482..4570150	-	669	WP_015139965.1	2OG-Fe dioxygenase family protein	-
NOS7524_RS18255	4570576..4571811	+	1236	WP_015136751.1	IS110 family transposase	-
NOS7524_RS18260	4572216..4572941	+	726	WP_015139966.1	DNA/RNA nuclease SfsA	-
NOS7524_RS18265	4572989..4573471	+	483	WP_015139967.1	gluconokinase	-
NOS7524_RS18270	4573527..4573823	+	297	WP_015139968.1	type II toxin-antitoxin system HicA family toxin	Toxin
NOS7524_RS18275	4573813..4574133	+	321	WP_015139969.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NOS7524_RS18280	4574240..4575211	-	972	WP_015139970.1	carbohydrate kinase	-
NOS7524_RS18285	4575577..4575840	+	264	WP_015139971.1	response regulator transcription factor	-
NOS7524_RS18290	4576315..4576977	+	663	WP_015139972.1	hypothetical protein	-
NOS7524_RS18295	4576984..4577985	+	1002	WP_015139973.1	glycosyltransferase	-
NOS7524_RS18300	4578146..4578769	-	624	WP_015139974.1	FMN-dependent NADH-azoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (25-75)

Antitoxin

• Uncharacterised protein family HicB (51-101)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11018.42 Da
• Isoelectric Point: 7.4040

>T28513 WP_015139968.1 NC_019684:4573527-4573823 [Nostoc sp. PCC 7524]
MELNSKHQKTLDDIFENPVRSNIAWSDIESMLVALGAEVSEGKGSRVRIALNGVRATFHRPHPEKETDKGAVKSMRRFLT
EANVKEYKHTGGLNDNEI

Nucleotide

• Length: 297 bp

>T28513 NC_019684:4573527-4573823 [Nostoc sp. PCC 7524]
ATGGAACTTAACAGTAAACATCAAAAAACACTTGATGACATTTTTGAAAATCCTGTTAGATCAAATATCGCTTGGAGTGA
TATTGAGTCTATGCTGGTTGCCCTTGGTGCAGAAGTGTCAGAAGGAAAGGGGTCAAGAGTAAGAATAGCTCTTAATGGTG
TCAGGGCGACTTTTCACAGACCACACCCAGAAAAAGAAACTGATAAAGGTGCGGTTAAGTCGATGCGGCGATTCTTAACA
GAAGCCAATGTTAAAGAATACAAGCACACAGGAGGACTCAACGACAATGAAATATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12346.99 Da
• Isoelectric Point: 4.8208

>AT28513 WP_015139969.1 NC_019684:4573813-4574133 [Nostoc sp. PCC 7524]
MKYKGYEAVVEFDDEAEIFHGEVINIRDVITFQGDSVKELKQAFYDSVDDYLEFCKERGEEPEKPFSGKFMLRINPELHK
TIAIKAKKEGQSLNSWIEKCLSRYAS

Nucleotide

• Length: 321 bp

>AT28513 NC_019684:4573813-4574133 [Nostoc sp. PCC 7524]
ATGAAATATAAAGGATATGAAGCAGTCGTTGAATTTGATGATGAAGCTGAAATTTTTCATGGCGAAGTAATTAATATTCG
AGATGTAATTACATTTCAAGGCGACAGCGTAAAAGAATTAAAGCAAGCCTTTTACGACTCAGTTGATGATTATTTAGAAT
TTTGTAAAGAACGTGGCGAAGAACCTGAAAAGCCATTTTCAGGTAAATTCATGCTCAGAATTAACCCTGAGTTACATAAA
ACAATCGCCATCAAAGCCAAGAAAGAAGGCCAAAGTCTTAATTCTTGGATAGAGAAATGCTTGAGTAGGTATGCGAGCTA
A

Structures

Toxin

• AlphaFold DB: K9QVA1

Antitoxin

• AlphaFold DB: K9QWL0