Detailed information of TA system TA028510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 4546381..4546949
• Replicon: chromosome
• Accession: NC_019683
• Organism: Leptolyngbya sp. PCC 7376

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: K9Q3A4
• Locus tag: LEPTO7376_RS20425
• Protein ID: WP_015135940.1
• Coordinates: 4546611..4546949 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: K9Q533
• Locus tag: LEPTO7376_RS20420
• Protein ID: WP_015135939.1
• Coordinates: 4546381..4546614 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEPTO7376_RS20420	4546381..4546614	+	234	WP_015135939.1	DUF433 domain-containing protein	Antitoxin
LEPTO7376_RS20425	4546611..4546949	+	339	WP_015135940.1	DUF5615 family PIN-like protein	Toxin
LEPTO7376_RS26655	4546994..4547143	-	150	WP_160148534.1	hypothetical protein	-
LEPTO7376_RS20430	4547342..4548001	-	660	WP_015135941.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-79)

Antitoxin

• Protein of unknown function DUF433 (11-66)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12515.45 Da
• Isoelectric Point: 4.5600

>T28510 WP_015135940.1 NC_019683:4546611-4546949 [Leptolyngbya sp. PCC 7376]
MKFWIDAQLPPSLAGWLSATFNVEAEALRDIGLRDAKDEDIFGAAQQQKNVVIMTKDSDFVDLVCRLGTPPQILWITCGN
VTNRNLRKLLLATFEQSLIQLRAGVNIIEISD

Nucleotide

• Length: 339 bp

>T28510 NC_019683:4546611-4546949 [Leptolyngbya sp. PCC 7376]
ATGAAGTTTTGGATAGATGCTCAACTTCCTCCCTCATTAGCAGGCTGGCTAAGTGCAACCTTCAATGTGGAAGCTGAGGC
TTTGAGAGATATTGGTTTGCGCGATGCGAAAGATGAGGACATCTTTGGTGCAGCTCAGCAACAAAAAAATGTAGTGATCA
TGACAAAAGACAGTGATTTTGTTGATCTTGTGTGTCGTTTAGGAACACCTCCTCAAATTCTTTGGATTACCTGTGGCAAT
GTGACTAACCGTAATCTTAGAAAATTACTGTTAGCTACTTTTGAACAGTCACTTATTCAACTTCGAGCCGGAGTAAATAT
TATCGAAATCAGTGACTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8574.94 Da
• Isoelectric Point: 4.6893

>AT28510 WP_015135939.1 NC_019683:4546381-4546614 [Leptolyngbya sp. PCC 7376]
MSKSNNLLSRITQTPGQCGGRPCIRGMRIRVCDILEMLAESVSADELLEDFPDLEFADIQACLIFAAKRTEFPRLTA

Nucleotide

• Length: 234 bp

>AT28510 NC_019683:4546381-4546614 [Leptolyngbya sp. PCC 7376]
ATGAGTAAATCGAATAATCTATTATCCCGTATCACCCAAACCCCTGGACAATGTGGCGGTCGCCCTTGCATTCGAGGCAT
GCGTATTCGTGTTTGTGACATCCTCGAAATGCTTGCTGAAAGTGTCAGTGCTGATGAGCTTTTAGAGGACTTTCCCGATC
TGGAGTTTGCAGATATTCAAGCTTGTCTGATTTTTGCGGCTAAACGAACTGAATTCCCTCGTCTAACAGCATGA

Structures

Toxin

• AlphaFold DB: K9Q3A4

Antitoxin

• AlphaFold DB: K9Q533