Detailed information of TA system TA028509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 4182142..4182713
• Replicon: chromosome
• Accession: NC_019683
• Organism: Leptolyngbya sp. PCC 7376

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: K9Q485
• Locus tag: LEPTO7376_RS18840
• Protein ID: WP_015135684.1
• Coordinates: 4182372..4182713 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: K9Q2K1
• Locus tag: LEPTO7376_RS18835
• Protein ID: WP_015135683.1
• Coordinates: 4182142..4182375 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LEPTO7376_RS18800	4177321..4177590	-	270	WP_160148515.1	hypothetical protein	-
LEPTO7376_RS18805	4177612..4178430	-	819	WP_015135678.1	IS6 family transposase	-
LEPTO7376_RS18810	4179120..4179473	+	354	WP_015135679.1	hypothetical protein	-
LEPTO7376_RS18815	4179470..4179868	+	399	WP_015135680.1	HNH endonuclease	-
LEPTO7376_RS25155	4180067..4180776	+	710	Protein_3742	IS1 family transposase	-
LEPTO7376_RS18830	4180914..4181978	+	1065	WP_015135682.1	phage integrase	-
LEPTO7376_RS18835	4182142..4182375	+	234	WP_015135683.1	DUF433 domain-containing protein	Antitoxin
LEPTO7376_RS18840	4182372..4182713	+	342	WP_015135684.1	DUF5615 family PIN-like protein	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-79)

Antitoxin

• Protein of unknown function DUF433 (11-66)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12550.38 Da
• Isoelectric Point: 4.1237

>T28509 WP_015135684.1 NC_019683:4182372-4182713 [Leptolyngbya sp. PCC 7376]
MKFWIDAQLPPSLAGWLSSTFDVEAEALRDIGLRDAKDEEIFGAAQEQANVVIMTKDSDFVDLVCRLGMPPQILWITCGN
VTNRNLRKLLSATFEQAVIQLQAGVNIVEISDQ

Nucleotide

• Length: 342 bp

>T28509 NC_019683:4182372-4182713 [Leptolyngbya sp. PCC 7376]
ATGAAATTTTGGATAGATGCTCAACTGCCACCCTCATTAGCAGGCTGGTTAAGTTCTACATTCGATGTGGAAGCTGAAGC
CTTGAGAGATATAGGTTTACGCGATGCTAAGGATGAAGAGATCTTTGGTGCAGCTCAAGAACAAGCCAATGTAGTCATCA
TGACGAAAGACAGCGATTTCGTTGATCTCGTTTGTCGTCTAGGAATGCCACCTCAAATTCTCTGGATTACTTGTGGCAAT
GTAACTAATCGCAATCTCAGAAAATTACTCTCTGCTACTTTTGAGCAGGCAGTCATCCAACTTCAGGCTGGTGTGAATAT
TGTCGAAATTAGTGACCAATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8589.96 Da
• Isoelectric Point: 4.4644

>AT28509 WP_015135683.1 NC_019683:4182142-4182375 [Leptolyngbya sp. PCC 7376]
MNTSNTLLSRITQTPGQCGGRPCIRGMRIRVCDILEMLAESVSVDELLEDFPDLEFADIQACLIFAAKRTEFPRLTA

Nucleotide

• Length: 234 bp

>AT28509 NC_019683:4182142-4182375 [Leptolyngbya sp. PCC 7376]
ATGAATACATCGAATACTTTACTCTCACGTATTACTCAAACCCCCGGTCAGTGTGGTGGTCGCCCATGCATAAGAGGTAT
GCGCATTCGAGTGTGTGACATCCTCGAAATGCTTGCTGAAAGTGTCAGTGTGGATGAACTGTTGGAAGACTTTCCCGATC
TGGAGTTTGCTGATATCCAAGCCTGTTTGATCTTTGCCGCTAAACGTACTGAATTTCCGCGTTTAACAGCATGA

Structures

Toxin

• AlphaFold DB: K9Q485

Antitoxin

• AlphaFold DB: K9Q2K1