Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3741550..3742204 Replicon chromosome
Accession NZ_HG738867
Organism Escherichia coli str. K-12 substr. MC4100 strain K-12

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag BN896_RS18530 Protein ID WP_000244777.1
Coordinates 3741797..3742204 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BN896_RS18525 Protein ID WP_000354046.1
Coordinates 3741550..3741816 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BN896_RS18500 (BN896_2627) 3736719..3737462 + 744 WP_000951964.1 SDR family oxidoreductase -
BN896_RS18505 (BN896_2626) 3737519..3738952 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
BN896_RS18510 (BN896_2625) 3738997..3739308 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
BN896_RS18515 (BN896_2624) 3739472..3740131 + 660 WP_000250274.1 hemolysin III family protein -
BN896_RS18520 (BN896_2623) 3740327..3741307 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BN896_RS18525 (BN896_2622) 3741550..3741816 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BN896_RS18530 (BN896_2621) 3741797..3742204 + 408 WP_000244777.1 toxin CptA Toxin
BN896_RS18535 (BN896_2620) 3742244..3742765 - 522 WP_001055874.1 flavodoxin FldB -
BN896_RS18540 (BN896_2619) 3742877..3743773 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BN896_RS18545 (BN896_2618) 3743798..3744508 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BN896_RS18550 (BN896_2617) 3744514..3746247 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T285028 WP_000244777.1 NZ_HG738867:3741797-3742204 [Escherichia coli str. K-12 substr. MC4100]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T285028 NZ_HG738867:3741797-3742204 [Escherichia coli str. K-12 substr. MC4100]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT285028 WP_000354046.1 NZ_HG738867:3741550-3741816 [Escherichia coli str. K-12 substr. MC4100]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT285028 NZ_HG738867:3741550-3741816 [Escherichia coli str. K-12 substr. MC4100]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References