Detailed information of TA system TA285015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1397815..1398453
• Replicon: chromosome
• Accession: NZ_HG738867
• Organism: Escherichia coli str. K-12 substr. MC4100 strain K-12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: BN896_RS06990
• Protein ID: WP_000813794.1
• Coordinates: 1397815..1397991 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BN896_RS06995
• Protein ID: WP_001270286.1
• Coordinates: 1398037..1398453 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN896_RS06970 (BN896_1258)	1393434..1394648	-	1215	WP_001326689.1	BenE family transporter YdcO	-
BN896_RS06975 (BN896_1259)	1394701..1395237	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
BN896_RS06980 (BN896_1260)	1395310..1397271	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
BN896_RS06985 (BN896_1261)	1397363..1397593	-	231	WP_000494244.1	YncJ family protein	-
BN896_RS06990 (BN896_1262)	1397815..1397991	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
BN896_RS06995 (BN896_1263)	1398037..1398453	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
BN896_RS07000 (BN896_1264)	1398532..1399938	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
BN896_RS07005 (BN896_1265)	1400183..1401328	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
BN896_RS07010 (BN896_1266)	1401346..1402359	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
BN896_RS07015 (BN896_1267)	1402360..1403301	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T285015 WP_000813794.1 NZ_HG738867:1397815-1397991 [Escherichia coli str. K-12 substr. MC4100]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T285015 NZ_HG738867:1397815-1397991 [Escherichia coli str. K-12 substr. MC4100]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT285015 WP_001270286.1 NZ_HG738867:1398037-1398453 [Escherichia coli str. K-12 substr. MC4100]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT285015 NZ_HG738867:1398037-1398453 [Escherichia coli str. K-12 substr. MC4100]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB