Detailed information of TA system TA028499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 699862..700459
• Replicon: chromosome
• Accession: NC_019680
• Organism: Synechococcus sp. PCC 6312

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K9RRN0
• Locus tag: SYN6312_RS03360
• Protein ID: WP_015123458.1
• Coordinates: 699862..700242 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9RPQ6
• Locus tag: SYN6312_RS03365
• Protein ID: WP_015123459.1
• Coordinates: 700235..700459 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SYN6312_RS03340	695463..696449	+	987	WP_015123454.1	glycosyltransferase family 4 protein	-
SYN6312_RS18680	696558..697958	-	1401	WP_015123455.1	ABC transporter ATP-binding protein	-
SYN6312_RS03350	697968..699188	-	1221	WP_015123456.1	ABC transporter permease	-
SYN6312_RS03355	699373..699618	-	246	WP_015123457.1	hypothetical protein	-
SYN6312_RS03360	699862..700242	-	381	WP_015123458.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SYN6312_RS03365	700235..700459	-	225	WP_015123459.1	hypothetical protein	Antitoxin
SYN6312_RS03370	700652..700996	-	345	WP_015123460.1	XisI protein	-
SYN6312_RS03375	700984..701400	-	417	WP_015123461.1	XisH family protein	-
SYN6312_RS03380	701537..701778	-	242	Protein_669	hypothetical protein	-
SYN6312_RS03385	702075..703577	-	1503	WP_015123464.1	TIGR04141 family sporadically distributed protein	-
SYN6312_RS03390	703873..704415	-	543	WP_015123465.1	DUF2721 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14235.33 Da
• Isoelectric Point: 7.1334

>T28499 WP_015123458.1 NC_019680:c700242-699862 [Synechococcus sp. PCC 6312]
MPNGNLTYKRGDIWWVDLKPVIGHETDKERPCLILQNDVGNQNGSTTIVAPLWPGTKTYPFVVNVVPTPQNGLDRERHIN
LSQMRAVDAQRIKNKQGVLEDVYWGEIEKAICIELGFSLAFKSFAI

Nucleotide

• Length: 381 bp

>T28499 NC_019680:c700242-699862 [Synechococcus sp. PCC 6312]
ATGCCTAACGGAAACTTAACCTACAAGCGAGGAGATATCTGGTGGGTTGATCTGAAACCTGTTATTGGCCATGAAACCGA
TAAAGAACGCCCTTGTCTAATTTTGCAAAATGATGTCGGCAATCAAAATGGTTCAACAACAATCGTTGCTCCCTTATGGC
CTGGAACAAAGACTTATCCGTTTGTTGTCAACGTTGTACCAACTCCCCAGAATGGACTCGATAGAGAGCGTCACATTAAC
TTAAGTCAAATGAGGGCTGTAGATGCTCAGAGAATTAAAAATAAACAGGGTGTTTTAGAAGATGTTTATTGGGGCGAAAT
CGAGAAGGCAATATGTATTGAACTTGGTTTCAGTTTAGCGTTTAAGTCCTTTGCAATATAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8484.47 Da
• Isoelectric Point: 4.1237

>AT28499 WP_015123459.1 NC_019680:c700459-700235 [Synechococcus sp. PCC 6312]
MSKKVSITLDDDVLDFVDRLASNRSSFINNVLWQEKRRILMKELEEAYKDQANDPELQEEISAWDVAVGDGLNA

Nucleotide

• Length: 225 bp

>AT28499 NC_019680:c700459-700235 [Synechococcus sp. PCC 6312]
ATGAGCAAAAAAGTCAGCATAACCTTAGATGACGACGTTCTGGATTTTGTCGATCGGCTCGCCAGCAACCGTAGCAGCTT
TATCAATAATGTCCTCTGGCAAGAGAAGAGAAGGATTTTGATGAAAGAGCTAGAAGAGGCTTATAAAGATCAGGCTAATG
ACCCAGAATTACAGGAAGAAATCTCGGCCTGGGATGTTGCAGTGGGTGATGGTTTAAATGCCTAA

Structures

Toxin

• AlphaFold DB: K9RRN0

Antitoxin

• AlphaFold DB: K9RPQ6