Detailed information of TA system TA284985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 4358664..4360105
• Replicon: chromosome
• Accession: NZ_HG322950
• Organism: Pseudomonas knackmussii B13

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A024HKC3
• Locus tag: PKB_RS20475
• Protein ID: WP_043254009.1
• Coordinates: 4359536..4360105 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: PKB_RS20470
• Protein ID: WP_043257565.1
• Coordinates: 4358664..4359512 (-)
• Length: 283 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PKB_RS20445	4353869..4354789	+	921	WP_043253999.1	recombination-associated protein RdgC	-
PKB_RS20450	4354860..4355798	+	939	WP_043254001.1	bile acid:sodium symporter family protein	-
PKB_RS20455	4355932..4356246	+	315	WP_043254003.1	quaternary ammonium compound efflux SMR transporter SugE	-
PKB_RS20460	4356406..4356615	+	210	WP_043254005.1	cold-shock protein	-
PKB_RS20465	4356664..4358538	-	1875	WP_043254007.1	MFS transporter	-
PKB_RS20470	4358664..4359512	-	849	WP_043257565.1	AraC family transcriptional regulator	Antitoxin
PKB_RS20475	4359536..4360105	-	570	WP_043254009.1	GNAT family N-acetyltransferase	Toxin
PKB_RS20480	4360359..4363838	+	3480	WP_043254012.1	hybrid sensor histidine kinase/response regulator	-
PKB_RS20485	4363850..4364308	-	459	WP_043254014.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-146)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-152)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21071.23 Da
• Isoelectric Point: 9.9885

>T284985 WP_043254009.1 NZ_HG322950:c4360105-4359536 [Pseudomonas knackmussii B13]
MFRPSPITLQRGALRLEPMVEADVSELVALAEANREALQFMSAPQRPDWYRQSIADQREGRALPLVVRLGNQIVGTTRFM
DFLPALPACEIGGTWLDSAQHGSGLNASIKYLMLRQAFDNWKMVRVQLKTAASNLRSQRAIEKLGAVREGVLRNHRRLAG
GRLDDSVIYSITDLEWPQVKAALEAGFNG

Nucleotide

• Length: 570 bp

Antitoxin

Protein

• Length: 283 a.a.
• Molecular weight: 31367.60 Da
• Isoelectric Point: 8.5298

>AT284985 WP_043257565.1 NZ_HG322950:c4359512-4358664 [Pseudomonas knackmussii B13]
MQDDAQGERTRFWRAQELGGLELLHARYIGQRFAPHVHEGFSLVLIEQGAQRFRHRGAEHLAPSGSLVLINPDEVHTGSK
AHDAGWQYRGFYPEIDQVGSVLDELSLGGRGLPTFDASVLHDPQLSRAFIHVHRLLESPASDLERQTCWREALLALFQRH
ARVPEARPYGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHLSRVFRQATGLPPHAWIKQRRLEQARALLKQGGA
PAMVAAQLGFADQSHLSRQFKQAYGVAPGEYRLACGRSIISS

Nucleotide

• Length: 849 bp

Structures

Toxin

• AlphaFold DB: A0A024HKC3