Detailed information of TA system TA028494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/MazF(toxin)
• Location: 1616140..1616714
• Replicon: chromosome
• Accession: NC_019676
• Organism: Nostoc sp. PCC 7107

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: -
• Locus tag: NOS7107_RS06920
• Protein ID: WP_157373984.1
• Coordinates: 1616370..1616714 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: K9Q9J3
• Locus tag: NOS7107_RS06915
• Protein ID: WP_015112259.1
• Coordinates: 1616140..1616376 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NOS7107_RS06880	1611399..1611713	-	315	WP_015112252.1	DUF2973 domain-containing protein	-
NOS7107_RS06885	1611977..1612294	-	318	WP_015112253.1	DUF2605 domain-containing protein	-
NOS7107_RS06890	1612650..1614491	+	1842	WP_015112254.1	threonine--tRNA ligase	-
NOS7107_RS06895	1614547..1614744	+	198	WP_015112255.1	hypothetical protein	-
NOS7107_RS06900	1614719..1615117	+	399	WP_015112256.1	XisH family protein	-
NOS7107_RS06905	1615105..1615440	+	336	WP_015112257.1	XisI protein	-
NOS7107_RS06910	1615576..1616043	+	468	WP_015112258.1	hypothetical protein	-
NOS7107_RS06915	1616140..1616376	+	237	WP_015112259.1	hypothetical protein	Antitoxin
NOS7107_RS06920	1616370..1616714	+	345	WP_157373984.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
NOS7107_RS06925	1616752..1616991	-	240	WP_015112261.1	hypothetical protein	-
NOS7107_RS06930	1617102..1618016	+	915	WP_015112262.1	homoserine kinase	-
NOS7107_RS06935	1618070..1618576	+	507	WP_015112263.1	hypothetical protein	-
NOS7107_RS06940	1618877..1620490	+	1614	WP_044499788.1	NAD(P)H-quinone oxidoreductase subunit 4	-
NOS7107_RS06945	1620710..1621090	-	381	WP_015112265.1	DUF423 domain-containing protein	-
NOS7107_RS06950	1621112..1621600	-	489	WP_015112266.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12712.64 Da
• Isoelectric Point: 4.2220

>T28494 WP_157373984.1 NC_019676:1616370-1616714 [Nostoc sp. PCC 7107]
MVMYRGEIWWANLPEPVGSEPGYRRPVLIIQDDTFNQSRISTVIVAVITSSIQLAEAPGNVLLSREVSGLPKDSVVNVSQ
LLTIDKSFLVERVGALPDYLQEEVDEGLRIILYL

Nucleotide

• Length: 345 bp

>T28494 NC_019676:1616370-1616714 [Nostoc sp. PCC 7107]
TTGGTAATGTATCGAGGTGAAATTTGGTGGGCGAACCTACCTGAGCCAGTAGGTTCTGAGCCTGGGTATCGTCGTCCTGT
TTTGATAATTCAAGATGATACGTTTAATCAAAGCCGCATCAGTACAGTCATAGTTGCTGTTATTACTTCAAGTATTCAAT
TGGCAGAAGCCCCAGGCAATGTATTGTTATCGCGTGAAGTATCAGGCTTACCTAAAGATTCTGTGGTTAACGTATCTCAA
CTTTTGACTATCGATAAAAGCTTTTTGGTTGAGCGAGTCGGAGCATTACCAGATTATTTGCAAGAGGAAGTAGATGAAGG
GCTACGAATAATTTTATACCTCTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8931.16 Da
• Isoelectric Point: 4.9901

>AT28494 WP_015112259.1 NC_019676:1616140-1616376 [Nostoc sp. PCC 7107]
MKTAISLPDSVFEEAEALATHLGISRSELYTKALQAYLQKYNRNQILSKLNQVYSQESAELDPVLAKMQFMSLPHEDW

Nucleotide

• Length: 237 bp

>AT28494 NC_019676:1616140-1616376 [Nostoc sp. PCC 7107]
ATGAAAACTGCTATTTCTCTACCAGATTCAGTTTTTGAAGAAGCAGAAGCACTTGCTACACATTTAGGAATATCACGCAG
TGAACTTTATACGAAAGCATTGCAAGCGTATCTGCAAAAGTATAACCGTAATCAAATTCTGAGCAAGCTGAACCAAGTTT
ATTCTCAAGAATCTGCTGAACTAGATCCAGTGTTGGCAAAGATGCAATTTATGTCTCTGCCACATGAGGATTGGTAA

Structures

Antitoxin

• AlphaFold DB: K9Q9J3