Detailed information of TA system TA028492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(antitoxin)
• Location: 1955280..1955848
• Replicon: chromosome
• Accession: NC_019675
• Organism: Cyanobium gracile PCC 6307

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CYAGR_RS09520
• Protein ID: WP_156818437.1
• Coordinates: 1955609..1955848 (-)
• Length: 80 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9P7Q2
• Locus tag: CYAGR_RS09515
• Protein ID: WP_015109590.1
• Coordinates: 1955280..1955621 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYAGR_RS09495	1950301..1951032	-	732	WP_015109586.1	hypothetical protein	-
CYAGR_RS09500	1951042..1951521	-	480	WP_015109587.1	VOC family protein	-
CYAGR_RS09505	1952001..1952693	+	693	WP_015109588.1	response regulator transcription factor	-
CYAGR_RS17815	1952830..1955271	+	2442	WP_015109589.1	glycoside hydrolase family 9 protein	-
CYAGR_RS09515	1955280..1955621	-	342	WP_015109590.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
CYAGR_RS09520	1955609..1955848	-	240	WP_156818437.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
CYAGR_RS09525	1955871..1956698	-	828	WP_015109592.1	CPBP family intramembrane metalloprotease	-
CYAGR_RS16540	1956816..1957388	+	573	WP_015109593.1	hypothetical protein	-
CYAGR_RS09535	1958162..1959817	+	1656	WP_015109594.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• mRNA interferase PemK-like (11-111)

Sequences

Toxin

Protein

• Length: 80 a.a.
• Molecular weight: 8333.46 Da
• Isoelectric Point: 4.5318

>T28492 WP_156818437.1 NC_019675:c1955848-1955609 [Cyanobium gracile PCC 6307]
VRAKLVRIGNSRGLRLAKPLLEEAGLTDEVDIHAAPGVLTITPVAAPRAGWAEAAAAVAPEGLLDPATPTHFDEGEWEW

Nucleotide

• Length: 240 bp

>T28492 NC_019675:c1955848-1955609 [Cyanobium gracile PCC 6307]
GTGAGGGCCAAGCTGGTCAGGATCGGCAATTCACGCGGTCTTCGTCTGGCCAAGCCGCTGCTCGAGGAAGCTGGCCTGAC
GGACGAGGTGGACATCCACGCCGCCCCTGGTGTGCTGACCATCACGCCTGTGGCGGCGCCGCGGGCCGGGTGGGCCGAGG
CCGCAGCAGCCGTGGCGCCGGAGGGTCTCCTGGATCCGGCCACTCCCACCCACTTCGACGAAGGGGAATGGGAGTGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12575.62 Da
• Isoelectric Point: 7.8886

>AT28492 WP_015109590.1 NC_019675:c1955621-1955280 [Cyanobium gracile PCC 6307]
MGVVTDVAVSRGDIFLVALNPTRGQEIRKTRPCVVVSPDELNAHMGTFIVAPLTTGAYPYPFRVPCSFQGKEGHVVPDQL
RTVDRERLVKRLGTLPNDTLHQLLTILQEMFAF

Nucleotide

• Length: 342 bp

>AT28492 NC_019675:c1955621-1955280 [Cyanobium gracile PCC 6307]
ATGGGAGTGGTGACGGACGTCGCCGTCAGCCGTGGTGACATCTTTCTGGTGGCGTTGAATCCCACCCGTGGCCAGGAGAT
CCGCAAGACCAGGCCCTGCGTGGTCGTGTCGCCCGATGAACTCAACGCCCACATGGGCACGTTCATCGTGGCGCCGTTGA
CGACCGGTGCTTATCCCTACCCCTTCCGCGTTCCTTGTTCGTTCCAGGGCAAAGAAGGCCACGTCGTCCCTGACCAGCTG
CGCACGGTTGATCGCGAACGGCTGGTGAAGCGGCTGGGAACCCTGCCGAACGACACGCTGCACCAGCTGCTGACCATCCT
TCAGGAGATGTTTGCGTTCTGA

Structures

Antitoxin

• AlphaFold DB: K9P7Q2