Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 524014..524591 Replicon chromosome
Accession NZ_FO704550
Organism Xenorhabdus doucetiae strain FRM16

Toxin (Protein)


Gene name mazF Uniprot ID A0A068QNH7
Locus tag XDD1_RS02615 Protein ID WP_045968421.1
Coordinates 524014..524346 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A068QR34
Locus tag XDD1_RS02620 Protein ID WP_045968422.1
Coordinates 524346..524591 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XDD1_RS18370 519402..520199 - 798 WP_052705631.1 DUF2829 domain-containing protein -
XDD1_RS02600 520521..521504 + 984 WP_045968417.1 IS30 family transposase -
XDD1_RS02605 521763..523052 - 1290 WP_045968418.1 bifunctional O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase -
XDD1_RS02610 523367..523945 + 579 WP_045968419.1 outer membrane beta-barrel protein -
XDD1_RS02615 524014..524346 - 333 WP_045968421.1 endoribonuclease MazF Toxin
XDD1_RS02620 524346..524591 - 246 WP_045968422.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
XDD1_RS02625 524782..525675 - 894 WP_045968425.1 Rpn family recombination-promoting nuclease/putative transposase -
XDD1_RS02630 526010..526384 - 375 WP_045968427.1 type II toxin-antitoxin system PemK/MazF family toxin -
XDD1_RS02635 526384..526656 - 273 WP_045968429.1 antitoxin -
XDD1_RS02640 526915..527703 + 789 WP_045968431.1 SDR family oxidoreductase -
XDD1_RS02645 527742..528986 + 1245 WP_045968433.1 hypothetical protein -
XDD1_RS02650 529035..529556 + 522 WP_148886033.1 flavin reductase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 520521..521504 983


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-109)

Antitoxin

Antitoxin PemI-like

(4-80)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11996.76 Da        Isoelectric Point: 7.2143

>T284891 WP_045968421.1 NZ_FO704550:c524346-524014 [Xenorhabdus doucetiae]
MVSRFVPDSGDIIWIDFDPVAGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKAKDYPFEVELSGSRDSIALADQVTCV
DWRARKVTKKGHVNSSELEDIKAKAKALIG

Download         Length: 333 bp

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8753.23 Da        Isoelectric Point: 4.6684

>AT284891 WP_045968422.1 NZ_FO704550:c524591-524346 [Xenorhabdus doucetiae]
MAQVVVKKWGNSPSVRLPVAIMQAASLNVDDTVDISVEDGRIIIIPVKAKEYSLDALLAGITPENIHEKMDFGPPVGKEL
I

Download         Length: 246 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A068QNH7

Antitoxin

Source ID Structure
AlphaFold DB A0A068QR34

References