Detailed information of TA system TA028489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1670814..1671442
• Replicon: chromosome
• Accession: NC_019675
• Organism: Cyanobium gracile PCC 6307

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: K9P655
• Locus tag: CYAGR_RS08060
• Protein ID: WP_015109307.1
• Coordinates: 1671065..1671442 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: K9P838
• Locus tag: CYAGR_RS08055
• Protein ID: WP_015109306.1
• Coordinates: 1670814..1671068 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYAGR_RS08020	1666079..1666738	+	660	WP_015109299.1	alpha/beta hydrolase	-
CYAGR_RS08025	1666801..1667955	-	1155	WP_015109300.1	ATP-binding protein	-
CYAGR_RS08030	1668070..1668558	-	489	WP_015109301.1	DUF4236 domain-containing protein	-
CYAGR_RS08035	1668711..1669202	-	492	WP_043325580.1	hypothetical protein	-
CYAGR_RS16460	1669293..1669613	-	321	WP_015109303.1	Rho termination factor N-terminal domain-containing protein	-
CYAGR_RS08045	1669597..1670094	-	498	WP_015109304.1	hypothetical protein	-
CYAGR_RS08050	1670237..1670707	+	471	WP_156818584.1	hypothetical protein	-
CYAGR_RS08055	1670814..1671068	+	255	WP_015109306.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CYAGR_RS08060	1671065..1671442	+	378	WP_015109307.1	PIN domain-containing protein	Toxin
CYAGR_RS08065	1671481..1671816	+	336	WP_015109308.1	hypothetical protein	-
CYAGR_RS08070	1671915..1672178	+	264	WP_015109309.1	hypothetical protein	-
CYAGR_RS08075	1672175..1672612	+	438	WP_015109310.1	type II toxin-antitoxin system VapC family toxin	-
CYAGR_RS08080	1672575..1673834	-	1260	WP_015109311.1	VWA domain-containing protein	-
CYAGR_RS16465	1673872..1674540	-	669	WP_015109312.1	MerR family transcriptional regulator	-
CYAGR_RS17760	1674704..1674979	+	276	WP_172637165.1	M28 family peptidase	-
CYAGR_RS18270	1674924..1675274	+	351	WP_172637166.1	hypothetical protein	-
CYAGR_RS08095	1675497..1676033	+	537	WP_015109313.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-114)

Antitoxin

• SpoVT-AbrB domain (9-43)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13565.61 Da
• Isoelectric Point: 6.7158

>T28489 WP_015109307.1 NC_019675:1671065-1671442 [Cyanobium gracile PCC 6307]
VIYLLDTNILFPLIKNRPPQVAGRIDQLADVDSLAMSFITWAELLRGAEGSQRREATLQQLDGPAICRHYAAQASALKRQ
ATPIGGNDLWIAAHALALGATLVSHNVREFARIEGLVLVDWAAPA

Nucleotide

• Length: 378 bp

>T28489 NC_019675:1671065-1671442 [Cyanobium gracile PCC 6307]
GTGATCTACCTGCTCGACACCAATATCCTTTTTCCCCTGATCAAGAACCGGCCGCCCCAGGTGGCTGGGCGCATCGATCA
GCTCGCCGATGTCGACAGCCTGGCGATGTCGTTCATCACCTGGGCCGAGCTGCTGCGCGGAGCCGAGGGCAGCCAGCGCC
GCGAGGCCACCCTCCAGCAGCTCGATGGCCCCGCCATCTGCCGCCACTACGCCGCGCAAGCCTCCGCCCTCAAGCGCCAG
GCCACCCCCATCGGCGGCAACGATCTCTGGATTGCGGCCCATGCCCTGGCCCTCGGCGCCACCCTGGTGAGCCACAACGT
GCGCGAATTCGCCCGCATCGAGGGCCTGGTCCTGGTGGATTGGGCGGCTCCTGCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9217.53 Da
• Isoelectric Point: 4.9305

>AT28489 WP_015109306.1 NC_019675:1670814-1671068 [Cyanobium gracile PCC 6307]
MDVLPSRVFMNGNSQAVRIPAEFRLATDRVQITRSPNGDLIIHPCPAQRGQALLDTLAGFEPSFLASLEAQQASPIPMQE
RESL

Nucleotide

• Length: 255 bp

>AT28489 NC_019675:1670814-1671068 [Cyanobium gracile PCC 6307]
ATGGACGTGCTTCCCAGCCGGGTGTTCATGAACGGCAACAGCCAGGCCGTGCGCATCCCCGCCGAGTTCCGCCTCGCCAC
CGACCGGGTGCAGATCACCCGCAGCCCCAACGGCGATCTGATCATTCACCCCTGCCCGGCCCAGCGGGGCCAGGCCCTGC
TGGACACCCTGGCCGGCTTCGAGCCCAGCTTCTTGGCCAGCCTGGAGGCGCAGCAGGCCAGTCCGATCCCGATGCAGGAG
CGGGAGAGCCTGTGA

Structures

Toxin

• AlphaFold DB: K9P655

Antitoxin

• AlphaFold DB: K9P838