Detailed information of TA system TA028488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1130822..1131405
• Replicon: chromosome
• Accession: NC_019675
• Organism: Cyanobium gracile PCC 6307

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CYAGR_RS05360
• Protein ID: WP_015108767.1
• Coordinates: 1130822..1131184 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: K9P5E2
• Locus tag: CYAGR_RS05365
• Protein ID: WP_015108768.1
• Coordinates: 1131184..1131405 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYAGR_RS05350	1127427..1128338	+	912	WP_015108764.1	hypothetical protein	-
CYAGR_RS16915	1128370..1129416	+	1047	WP_083891349.1	glycosyltransferase family 61 protein	-
CYAGR_RS05355	1129497..1130813	-	1317	WP_015108766.1	hypothetical protein	-
CYAGR_RS05360	1130822..1131184	-	363	WP_015108767.1	type II toxin-antitoxin system VapC family toxin	Toxin
CYAGR_RS05365	1131184..1131405	-	222	WP_015108768.1	ribbon-helix-helix domain-containing protein	Antitoxin
CYAGR_RS05370	1131418..1131711	-	294	WP_156818391.1	hypothetical protein	-
CYAGR_RS17720	1131746..1133689	-	1944	WP_043325462.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-107)

Antitoxin

• Ribbon-helix-helix protein, CopG (7-40)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13212.50 Da
• Isoelectric Point: 4.8305

>T28488 WP_015108767.1 NC_019675:c1131184-1130822 [Cyanobium gracile PCC 6307]
MGLLLDSNILIDVLLGREVALRWIQAQPPCSISVITWIEVLVGCGPGEGEAVEVWLRGFHFLPLDGAVAREAVDCRRVLG
LKVPDAIILATARCHQLRLATRNTRDFPEELADVVVPYTL

Nucleotide

• Length: 363 bp

>T28488 NC_019675:c1131184-1130822 [Cyanobium gracile PCC 6307]
ATGGGACTGCTGCTCGACTCCAACATCCTGATCGATGTGTTGCTGGGCAGGGAGGTTGCCTTGCGGTGGATCCAGGCCCA
GCCCCCCTGTTCGATCAGCGTGATCACTTGGATCGAGGTGCTGGTGGGTTGCGGACCGGGCGAAGGCGAGGCGGTGGAGG
TGTGGTTGCGGGGATTCCACTTCCTGCCGCTCGATGGTGCCGTGGCCCGGGAGGCGGTGGACTGCCGTCGCGTCCTGGGG
TTGAAGGTGCCGGACGCCATCATCCTGGCCACCGCCCGTTGCCATCAGTTGCGGCTGGCCACGCGCAACACCCGGGATTT
TCCGGAGGAGCTGGCCGACGTGGTTGTCCCCTACACCCTCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8682.81 Da
• Isoelectric Point: 5.8407

>AT28488 WP_015108768.1 NC_019675:c1131405-1131184 [Cyanobium gracile PCC 6307]
MRTIVDLPEGERDQLDALCSQRGISRAQALREALILWLERERPRHADVFGLWSDRDLDGVALQRRIRQEWDQR

Nucleotide

• Length: 222 bp

>AT28488 NC_019675:c1131405-1131184 [Cyanobium gracile PCC 6307]
ATGCGCACCATCGTGGATCTGCCCGAGGGCGAGCGTGATCAGCTGGACGCCCTCTGTTCCCAGAGGGGCATTTCCCGGGC
TCAGGCGCTCCGGGAGGCCCTGATCCTCTGGCTGGAGCGGGAGCGTCCACGCCACGCGGACGTGTTCGGACTCTGGAGCG
ATCGGGACCTCGACGGGGTGGCTCTGCAGCGGCGGATCCGGCAGGAGTGGGACCAGCGCTGA

Structures

Antitoxin

• AlphaFold DB: K9P5E2