Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 481330..481967 Replicon chromosome
Accession NZ_CP128551
Organism Bacillus velezensis strain SRCM123441

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QTN52_RS02435 Protein ID WP_003156187.1
Coordinates 481617..481967 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag QTN52_RS02430 Protein ID WP_003156188.1
Coordinates 481330..481611 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTN52_RS02410 (477695) 477695..478294 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
QTN52_RS02415 (478387) 478387..478752 + 366 WP_014304402.1 holo-ACP synthase -
QTN52_RS02420 (478917) 478917..479924 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
QTN52_RS02425 (480041) 480041..481210 + 1170 WP_104843106.1 alanine racemase -
QTN52_RS02430 (481330) 481330..481611 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QTN52_RS02435 (481617) 481617..481967 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QTN52_RS02440 (482085) 482085..482906 + 822 WP_003156182.1 STAS domain-containing protein -
QTN52_RS02445 (482911) 482911..483276 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
QTN52_RS02450 (483279) 483279..483680 + 402 WP_134986389.1 anti-sigma regulatory factor -
QTN52_RS02455 (483692) 483692..484699 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
QTN52_RS02460 (484763) 484763..485092 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
QTN52_RS02465 (485089) 485089..485571 + 483 WP_007609591.1 anti-sigma B factor RsbW -
QTN52_RS02470 (485537) 485537..486325 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
QTN52_RS02475 (486325) 486325..486927 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T284877 WP_003156187.1 NZ_CP128551:481617-481967 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT284877 WP_003156188.1 NZ_CP128551:481330-481611 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References