Detailed information of TA system TA028484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1589900..1590464
• Replicon: chromosome
• Accession: NC_019673
• Organism: Saccharothrix espanaensis DSM 44229

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K0JVN9
• Locus tag: BN6_RS07565
• Protein ID: WP_015098977.1
• Coordinates: 1590117..1590464 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BN6_RS07560
• Protein ID: WP_041312224.1
• Coordinates: 1589900..1590130 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN6_RS43345	1585717..1586176	-	460	Protein_1509	carboxymuconolactone decarboxylase family protein	-
BN6_RS07545	1586364..1587332	-	969	Protein_1510	LCP family protein	-
BN6_RS07550	1587384..1587788	+	405	WP_015098973.1	hypothetical protein	-
BN6_RS07555	1587871..1589817	+	1947	WP_015098975.1	glycosyltransferase	-
BN6_RS07560	1589900..1590130	+	231	WP_041312224.1	ribbon-helix-helix domain-containing protein	Antitoxin
BN6_RS07565	1590117..1590464	+	348	WP_015098977.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BN6_RS07570	1590550..1590741	-	192	WP_015098978.1	ferredoxin	-
BN6_RS07575	1590741..1591937	-	1197	WP_015098979.1	cytochrome P450	-
BN6_RS07580	1592172..1593116	+	945	WP_085983586.1	ABC transporter ATP-binding protein	-
BN6_RS07585	1593113..1593967	+	855	WP_015098981.1	ABC transporter permease	-
BN6_RS07590	1593964..1594203	+	240	WP_015098982.1	helix-turn-helix transcriptional regulator	-
BN6_RS07595	1594787..1595422	-	636	WP_015098983.1	TetR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12221.17 Da
• Isoelectric Point: 10.5381

>T28484 WP_015098977.1 NC_019673:1590117-1590464 [Saccharothrix espanaensis DSM 44229]
MRRGDIYWVDLEPSVGAEANKIRPAVVVSNDAANRAAGRGGRGVVTIVPITSNTARVYPFQVLLPAAECGLGADSKAQAE
QVRTIAAARLRARIGELPPAVLKKLEEALRVHLAL

Nucleotide

• Length: 348 bp

>T28484 NC_019673:1590117-1590464 [Saccharothrix espanaensis DSM 44229]
ATGCGTCGAGGTGACATCTACTGGGTCGATCTGGAGCCGTCCGTCGGTGCGGAGGCGAACAAGATCAGACCGGCCGTCGT
CGTCAGCAACGACGCGGCGAACCGGGCCGCCGGGCGGGGCGGGCGGGGTGTCGTGACCATCGTGCCGATCACCTCGAACA
CCGCTCGGGTGTACCCGTTCCAGGTCCTGCTGCCGGCCGCGGAGTGCGGGCTGGGCGCGGACTCGAAGGCGCAGGCCGAG
CAGGTGCGGACGATCGCGGCGGCGCGGTTGCGCGCCCGGATCGGGGAGCTGCCGCCCGCGGTGCTCAAGAAGCTCGAAGA
GGCGTTGCGGGTGCACCTCGCGCTGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8518.30 Da
• Isoelectric Point: 4.0410

>AT28484 WP_041312224.1 NC_019673:1589900-1590130 [Saccharothrix espanaensis DSM 44229]
VKISVSLPEEDVEFVDRYLLQHRSASRSSVIHQAIALLRESGLENAYANAWDEWEADGESEMWDVTAPDGVVDASR

Nucleotide

• Length: 231 bp

>AT28484 NC_019673:1589900-1590130 [Saccharothrix espanaensis DSM 44229]
GTGAAAATCAGTGTGAGTCTGCCGGAGGAAGACGTAGAGTTTGTTGATCGCTACCTTCTGCAACATCGCAGTGCCTCACG
GTCGTCGGTGATCCACCAGGCGATCGCGTTGTTGCGGGAGTCCGGGTTGGAGAACGCCTACGCGAACGCGTGGGACGAGT
GGGAAGCCGACGGTGAATCCGAGATGTGGGACGTGACTGCGCCAGACGGTGTTGTCGATGCGTCGAGGTGA

Structures

Toxin

• AlphaFold DB: K0JVN9