Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 2040376..2041415 Replicon chromosome
Accession NZ_CP128540
Organism Pseudomonas alloputida strain NMI24_14

Toxin (Protein)


Gene name PP_4152 Uniprot ID Q88FE5
Locus tag LU682_RS09345 Protein ID WP_010954922.1
Coordinates 2040376..2040951 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A2V4F8J0
Locus tag LU682_RS09350 Protein ID WP_049588231.1
Coordinates 2040948..2041415 (-) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU682_RS09335 (LU682_009335) 2036005..2037228 + 1224 WP_010954925.1 NAD(P)/FAD-dependent oxidoreductase -
LU682_RS09340 (LU682_009340) 2037232..2040162 - 2931 WP_049588229.1 aminotransferase -
LU682_RS09345 (LU682_009345) 2040376..2040951 - 576 WP_010954922.1 PIN domain-containing protein Toxin
LU682_RS09350 (LU682_009350) 2040948..2041415 - 468 WP_049588231.1 helix-turn-helix domain-containing protein Antitoxin
LU682_RS09355 (LU682_009355) 2041563..2043167 - 1605 WP_010954920.1 ABC transporter ATP-binding protein -
LU682_RS09360 (LU682_009360) 2043169..2044188 - 1020 WP_010954919.1 ABC transporter permease -
LU682_RS09365 (LU682_009365) 2044190..2045263 - 1074 WP_010954918.1 microcin C ABC transporter permease YejB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

Helix-turn-helix domain, group 17

(84-132)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21646.86 Da        Isoelectric Point: 5.5710

>T284834 WP_010954922.1 NZ_CP128540:c2040951-2040376 [Pseudomonas alloputida]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFSKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHSVQQYLDRLLQQKLPETVKLLSAFKFLL

Download         Length: 576 bp

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17112.54 Da        Isoelectric Point: 6.3403

>AT284834 WP_049588231.1 NZ_CP128540:c2041415-2040948 [Pseudomonas alloputida]
MTTANLSSHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRAAIDALAAQAQELKMGYE

Download         Length: 468 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88FE5

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4F8J0

References