Detailed information of TA system TA028479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4053811..4054327
• Replicon: chromosome
• Accession: NC_019670
• Organism: Pseudomonas sp. UW4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: K9NM29
• Locus tag: PPUTUW4_RS17745
• Protein ID: WP_015095930.1
• Coordinates: 4054040..4054327 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: K9NKR6
• Locus tag: PPUTUW4_RS17740
• Protein ID: WP_015095929.1
• Coordinates: 4053811..4054050 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPUTUW4_RS17715	4048818..4049543	+	726	WP_015095924.1	murein L,D-transpeptidase catalytic domain family protein	-
PPUTUW4_RS17720	4049624..4051315	+	1692	WP_015095925.1	copper resistance system multicopper oxidase	-
PPUTUW4_RS17725	4051326..4052228	+	903	WP_015095926.1	copper resistance protein B	-
PPUTUW4_RS17730	4052281..4052664	+	384	WP_015095927.1	copper homeostasis periplasmic binding protein CopC	-
PPUTUW4_RS17735	4052668..4053591	+	924	WP_015095928.1	copper homeostasis membrane protein CopD	-
PPUTUW4_RS17740	4053811..4054050	+	240	WP_015095929.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
PPUTUW4_RS17745	4054040..4054327	+	288	WP_015095930.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PPUTUW4_RS17750	4054479..4055240	+	762	WP_086780454.1	transporter substrate-binding domain-containing protein	-
PPUTUW4_RS17755	4055471..4056466	+	996	WP_015095932.1	tRNA dihydrouridine(20/20a) synthase DusA	-
PPUTUW4_RS17760	4056645..4057571	+	927	WP_007978614.1	transaldolase	-
PPUTUW4_RS17765	4057856..4058338	-	483	WP_007978612.1	STAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11298.14 Da
• Isoelectric Point: 10.4047

>T28479 WP_015095930.1 NC_019670:4054040-4054327 [Pseudomonas sp. UW4]
MTYSLEFDARALKEWQKLGDTVRQQLKRKLVEILNNPRIEANRLHGFPDCYKVKLRSSGYRLVYQIIDQEITVFVVAVDK
REREQVYRKAAGRLK

Nucleotide

• Length: 288 bp

>T28479 NC_019670:4054040-4054327 [Pseudomonas sp. UW4]
ATGACCTATAGCCTTGAGTTTGATGCCCGTGCATTGAAGGAATGGCAAAAACTTGGCGACACAGTCCGACAGCAGCTCAA
AAGGAAGCTGGTGGAGATCCTGAACAATCCACGCATTGAAGCTAACCGCCTGCATGGTTTTCCCGACTGCTACAAGGTCA
AACTACGTAGCAGCGGTTACCGCTTGGTCTATCAAATAATTGACCAGGAAATTACCGTGTTTGTGGTAGCCGTGGATAAA
CGGGAACGTGAGCAGGTTTATCGCAAGGCGGCTGGACGCTTGAAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8983.37 Da
• Isoelectric Point: 4.5881

>AT28479 WP_015095929.1 NC_019670:4053811-4054050 [Pseudomonas sp. UW4]
MASINIRIDDDLKARAYLELEKLGVTPSELMRQALQYVAERGQLPFKPVLMTEDDEALLATVRERLASPQRVRVSIDDL

Nucleotide

• Length: 240 bp

>AT28479 NC_019670:4053811-4054050 [Pseudomonas sp. UW4]
ATGGCATCTATCAACATCCGTATCGATGACGACCTCAAAGCTCGCGCCTATCTCGAACTGGAAAAGCTCGGCGTCACCCC
TTCCGAACTGATGCGCCAAGCCCTGCAATATGTGGCGGAGCGTGGTCAGTTGCCTTTCAAGCCTGTCTTGATGACTGAAG
ACGATGAGGCTCTATTGGCCACTGTGCGGGAACGGCTTGCATCTCCTCAACGAGTAAGGGTTTCTATAGATGACCTATAG

Structures

Toxin

• AlphaFold DB: K9NM29

Antitoxin

• AlphaFold DB: K9NKR6