Detailed information of TA system TA284780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1758483..1759079
• Replicon: chromosome
• Accession: NZ_CP128526
• Organism: Mannheimia haemolytica strain 171117

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: QTV59_RS09410
• Protein ID: WP_006249776.1
• Coordinates: 1758483..1758707 (+)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A547EKP0
• Locus tag: QTV59_RS09415
• Protein ID: WP_006249777.1
• Coordinates: 1758750..1759079 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QTV59_RS09390	1754364..1755266	-	903	WP_006249771.1	DMT family transporter	-
QTV59_RS09395	1755263..1755619	-	357	WP_006249772.1	dihydroneopterin aldolase	-
QTV59_RS09400	1755689..1756294	+	606	WP_006249773.1	glycerol-3-phosphate 1-O-acyltransferase PlsY	-
QTV59_RS09405	1756368..1758275	-	1908	WP_006249774.1	DNA topoisomerase IV subunit B	-
QTV59_RS09410	1758483..1758707	+	225	WP_006249776.1	type II toxin-antitoxin system HicA family toxin	Toxin
QTV59_RS09415	1758750..1759079	+	330	WP_006249777.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
QTV59_RS09420	1759118..1759504	-	387	WP_006249778.1	Cu(I)-responsive transcriptional regulator	-
QTV59_RS09425	1759593..1759793	+	201	WP_006249779.1	heavy metal-associated domain-containing protein	-
QTV59_RS09430	1759846..1762038	+	2193	WP_006249780.1	copper-translocating P-type ATPase	-
QTV59_RS09435	1762091..1762876	-	786	WP_006249781.1	tRNA pseudouridine(38-40) synthase TruA	-
QTV59_RS09440	1762941..1763630	-	690	WP_006253589.1	GTP cyclohydrolase I FolE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (28-73)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 10.5597

>T284780 WP_006249776.1 NZ_CP128526:1758483-1758707 [Mannheimia haemolytica]
MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEEREGSRIAVVLFQQVRVFHRPHPNPDTDKGR

Nucleotide

• Length: 225 bp

>T284780 NZ_CP128526:1758483-1758707 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12312.06 Da
• Isoelectric Point: 4.7022

>AT284780 WP_006249777.1 NZ_CP128526:1758750-1759079 [Mannheimia haemolytica]
MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQLKKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSP
ELHKAAVIAATAENLSLNEWINQTLEKSV

Nucleotide

• Length: 330 bp

>AT284780 NZ_CP128526:1758750-1759079 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A547EKP0