Detailed information of TA system TA028475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 281009..281646
• Replicon: chromosome
• Accession: NC_019670
• Organism: Pseudomonas sp. UW4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: K9NBP0
• Locus tag: PPUTUW4_RS01290
• Protein ID: WP_015093063.1
• Coordinates: 281009..281416 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: K9NCY5
• Locus tag: PPUTUW4_RS01295
• Protein ID: WP_015093064.1
• Coordinates: 281416..281646 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPUTUW4_RS01270	276678..277373	-	696	WP_015093060.1	ABC transporter permease	-
PPUTUW4_RS01275	277446..278195	-	750	WP_015093061.1	ABC transporter substrate-binding protein	-
PPUTUW4_RS01280	278209..278982	-	774	WP_007972784.1	ABC transporter ATP-binding protein	-
PPUTUW4_RS01285	279547..280938	+	1392	WP_015093062.1	GABA permease	-
PPUTUW4_RS01290	281009..281416	-	408	WP_015093063.1	tRNA(fMet)-specific endonuclease VapC	Toxin
PPUTUW4_RS01295	281416..281646	-	231	WP_015093064.1	antitoxin	Antitoxin
PPUTUW4_RS01300	281816..282271	-	456	WP_015093065.1	hypothetical protein	-
PPUTUW4_RS01305	282278..282694	-	417	WP_007995339.1	hypothetical protein	-
PPUTUW4_RS01310	282731..283705	-	975	WP_015093066.1	alpha/beta fold hydrolase	-
PPUTUW4_RS01315	283774..284832	-	1059	WP_015093067.1	DUF1016 family protein	-
PPUTUW4_RS01320	285087..285536	+	450	WP_015093068.1	hypothetical protein	-
PPUTUW4_RS01325	285567..286373	-	807	WP_015093069.1	TSUP family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15090.48 Da
• Isoelectric Point: 6.9689

>T28475 WP_015093063.1 NC_019670:c281416-281009 [Pseudomonas sp. UW4]
MIKYMLDTNICIFTIKNKPQAVREAFNRHHGQLCISAITLMELIYGAEKSAAPEKNLAVIEGFAARLEVLPFDNEAAAHT
AMIRSELAKAGTPIGPYDQMIAGHARSRGFIVVTNNLREFERVPGLRVEDWVHIE

Nucleotide

• Length: 408 bp

>T28475 NC_019670:c281416-281009 [Pseudomonas sp. UW4]
ATGATCAAGTACATGCTCGATACCAACATCTGCATCTTCACCATCAAGAACAAACCACAGGCGGTACGGGAGGCGTTCAA
CCGTCATCACGGCCAACTGTGCATCAGTGCGATAACGCTGATGGAACTGATCTACGGAGCGGAAAAGTCAGCAGCACCCG
AAAAAAACCTGGCGGTCATCGAGGGCTTCGCTGCCCGACTTGAAGTCCTGCCCTTCGACAACGAAGCAGCAGCGCACACC
GCAATGATTCGATCCGAACTGGCAAAGGCCGGCACTCCCATCGGCCCGTACGACCAAATGATTGCCGGGCACGCACGCTC
ACGCGGGTTTATCGTGGTCACCAACAATCTGCGGGAGTTTGAGCGCGTTCCGGGGTTACGTGTAGAAGATTGGGTTCACA
TCGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8567.56 Da
• Isoelectric Point: 4.3867

>AT28475 WP_015093064.1 NC_019670:c281646-281416 [Pseudomonas sp. UW4]
MEQTTLFMSNRSQAVRLSKAVAMPSDVKRVDVVAVGRARIITPAGETWDSWFDGESVTADFMTDREQPAEQERESF

Nucleotide

• Length: 231 bp

>AT28475 NC_019670:c281646-281416 [Pseudomonas sp. UW4]
ATGGAACAGACAACCCTTTTCATGAGCAATCGAAGCCAGGCCGTTCGCCTGTCAAAAGCCGTGGCCATGCCAAGCGACGT
GAAACGGGTCGACGTGGTCGCCGTAGGCAGAGCCCGCATCATCACGCCCGCAGGTGAAACCTGGGACAGTTGGTTCGATG
GCGAAAGCGTCACCGCAGATTTCATGACCGATCGCGAGCAACCCGCCGAACAGGAGCGTGAGTCGTTCTGA

Structures

Toxin

• AlphaFold DB: K9NBP0

Antitoxin

• AlphaFold DB: K9NCY5