Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 253359..253948 Replicon chromosome
Accession NZ_CP128522
Organism Mannheimia haemolytica strain 182963

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag QTV63_RS01275 Protein ID WP_006248516.1
Coordinates 253359..253691 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag QTV63_RS01280 Protein ID WP_006248517.1
Coordinates 253688..253948 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTV63_RS01235 248554..249174 - 621 WP_006248508.1 dTMP kinase -
QTV63_RS01240 249185..250219 - 1035 WP_006248509.1 endolytic transglycosylase MltG -
QTV63_RS01260 250799..251326 + 528 WP_006248512.1 inorganic diphosphatase -
QTV63_RS01265 251382..252137 + 756 WP_006248513.1 M48 family metallopeptidase -
QTV63_RS01270 252201..253232 - 1032 WP_061887288.1 tryptophan--tRNA ligase -
QTV63_RS01275 253359..253691 - 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QTV63_RS01280 253688..253948 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
QTV63_RS01285 254018..254686 - 669 WP_006248518.1 phosphoglycolate phosphatase -
QTV63_RS01290 254773..255570 - 798 WP_021279892.1 prolipoprotein diacylglyceryl transferase -
QTV63_RS01295 255578..256366 - 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
QTV63_RS01300 256368..257012 - 645 WP_006248521.1 RNA pyrophosphohydrolase -
QTV63_RS01305 257624..258769 + 1146 WP_006251102.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T284745 WP_006248516.1 NZ_CP128522:c253691-253359 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT284745 WP_006248517.1 NZ_CP128522:c253948-253688 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References