Detailed information of TA system TA028474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 148078..148594
• Replicon: chromosome
• Accession: NC_019670
• Organism: Pseudomonas sp. UW4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: J2YKB6
• Locus tag: PPUTUW4_RS00685
• Protein ID: WP_007975642.1
• Coordinates: 148313..148594 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J2QY16
• Locus tag: PPUTUW4_RS00680
• Protein ID: WP_007975643.1
• Coordinates: 148078..148323 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPUTUW4_RS00655	143973..145520	+	1548	WP_041474759.1	efflux transporter outer membrane subunit	-
PPUTUW4_RS00660	145656..146414	-	759	WP_015092956.1	slipin family protein	-
PPUTUW4_RS00665	146417..146950	-	534	WP_015092957.1	hypothetical protein	-
PPUTUW4_RS00670	147150..147611	+	462	WP_007975646.1	YbaK/EbsC family protein	-
PPUTUW4_RS00675	147750..147995	+	246	WP_015092958.1	DUF2789 domain-containing protein	-
PPUTUW4_RS00680	148078..148323	+	246	WP_007975643.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
PPUTUW4_RS00685	148313..148594	+	282	WP_007975642.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PPUTUW4_RS00690	148727..149155	+	429	WP_041474760.1	winged helix DNA-binding protein	-
PPUTUW4_RS00695	149152..151224	+	2073	WP_041474761.1	FUSC family protein	-
PPUTUW4_RS00700	151221..151430	+	210	WP_007975639.1	DUF1656 domain-containing protein	-
PPUTUW4_RS00705	151427..152314	+	888	WP_015092961.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-71)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11082.90 Da
• Isoelectric Point: 10.3652

>T28474 WP_007975642.1 NC_019670:148313-148594 [Pseudomonas sp. UW4]
MTYKLEFLPSARKEWDKLGHTVREQFKKKLSERLEVPRIQSDALHGMPDCYKIKLRSSGYRLVYEVIDERIVVSVVAVGK
RERSEVYERARKR

Nucleotide

• Length: 282 bp

>T28474 NC_019670:148313-148594 [Pseudomonas sp. UW4]
ATGACTTATAAGCTCGAATTTCTCCCGTCTGCACGCAAAGAATGGGACAAGCTGGGGCACACAGTACGTGAGCAATTCAA
AAAGAAGCTGTCAGAGCGGCTTGAGGTGCCTCGAATCCAGTCTGACGCGCTGCATGGCATGCCTGACTGCTACAAGATCA
AGCTGAGGTCATCGGGATATCGACTCGTCTACGAGGTGATAGATGAGCGCATTGTTGTTTCTGTCGTTGCAGTAGGAAAG
CGCGAACGCAGTGAGGTGTATGAGCGCGCCAGGAAACGTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8896.26 Da
• Isoelectric Point: 4.7124

>AT28474 WP_007975643.1 NC_019670:148078-148323 [Pseudomonas sp. UW4]
MQNVLADMAVSVSELKKNPSAVLNGAHGGPVAVLNHNRVMGYMVPADVFEAMMERLDDLELAELVRSRRHETPIPVTLDD
L

Nucleotide

• Length: 246 bp

>AT28474 NC_019670:148078-148323 [Pseudomonas sp. UW4]
ATGCAAAATGTTTTGGCCGATATGGCTGTCAGCGTGTCTGAACTAAAGAAAAACCCCTCTGCCGTTTTGAACGGCGCCCA
TGGAGGGCCTGTCGCGGTGCTCAATCACAATCGCGTCATGGGCTACATGGTTCCTGCCGATGTGTTCGAAGCCATGATGG
AGCGCCTTGATGATTTGGAGTTGGCGGAACTCGTGCGTTCTCGTCGTCATGAAACGCCGATCCCGGTAACGCTGGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: J2YKB6

Antitoxin

• AlphaFold DB: J2QY16